Gasdermin B (GSDMB) in psoriatic patients-a preliminary comprehensive study on human serum, urine and skin

Julia Nowowiejska; Anna Baran; Anna Pryczynicz; Justyna Magdalena Hermanowicz; Beata Sieklucka; Dariusz Pawlak; Iwona Flisiak

doi:10.3389/fmolb.2024.1382069

Gasdermin B (GSDMB) in psoriatic patients-a preliminary comprehensive study on human serum, urine and skin

Front Mol Biosci. 2024 Apr 17:11:1382069. doi: 10.3389/fmolb.2024.1382069. eCollection 2024.

Authors

Julia Nowowiejska¹, Anna Baran¹, Anna Pryczynicz², Justyna Magdalena Hermanowicz³, Beata Sieklucka³, Dariusz Pawlak³, Iwona Flisiak¹

Affiliations

¹ Department of Dermatology and Venereology, Medical University of Bialystok, Bialystok, Poland.
² Department of General Pathomorphology, Medical University of Bialystok, Bialystok, Poland.
³ Department of Pharmacodynamics, Medical University of Bialystok, Bialystok, Poland.

Abstract

Psoriasis is one of the most common skin diseases and a crucial issue to manage in contemporary dermatology. The search for the details of its pathogenesis, markers and treatment is continuously ongoing. Our aim was to investigate the role of gasdermin B (GSDMB) in psoriasis, the second protein from the gasdermin family, involved in cell death and proliferation. GSDMB serum and urinary concentrations have never been studied in psoriatics, neither tissue expression of GSDMB by immunohistochemistry. The study included 60 psoriatic patients and 30 volunteers without dermatoses as controls. The serum and urinary GSDMB were evaluated by ELISA. Tissue expression of GSDMB was analyzed by immunohistochemistry. The serum and absolute urine concentrations of GSDMB were significantly higher in psoriatic patients than controls without skin diseases (p = 0.0137, p = 0.039 respectively). Urinary GSDMB/creatinine concentration ratio was significantly lower in patients compared to controls (p = 0.0241). The expression of GSDMB in the dermis and epidermis was significantly more prevalent in psoriatic plaque compared to the non-lesional skin and healthy skin of controls (p = 0.0012, p = 0.017, respectively). Serum GSDMB correlated positively with the age of patients (R = 0.41; p = 0.001). Our study adds to the current state of knowledge about psoriasis concerning the potential involvement of GSDMB. Possibly it could be engaged in keratinocytes migration, which requires further research. Elevated serum GSDMB and decreased urinary GSDMB/creatinine concentration ratio could potentially be investigated as psoriasis biomarkers. GSDMB could be investigated in the future as a potential therapeutic target.

Keywords: GSDMB; cell migration; gasdermin B; keratinocytes; psoriasis; pyroptosis.

Grants and funding

The author(s) declare that financial support was received for the research, authorship, and/or publication of this article. The part of the study involving the assessment of serum and urinary GSDMB was funded by the National Science Center, Poland, no NCN/1/MI/22/001/1149. The part of the study involving the assessment of tissue expression of GSDMB was funded by the Medical University of Bialystok, Poland, no B.SUB.23.358.