Temporal dynamics of the multi-omic response to endurance exercise training

Nature. 2024 May;629(8010):174-183. doi: 10.1038/s41586-023-06877-w. Epub 2024 May 1.

Abstract

Regular exercise promotes whole-body health and prevents disease, but the underlying molecular mechanisms are incompletely understood1-3. Here, the Molecular Transducers of Physical Activity Consortium4 profiled the temporal transcriptome, proteome, metabolome, lipidome, phosphoproteome, acetylproteome, ubiquitylproteome, epigenome and immunome in whole blood, plasma and 18 solid tissues in male and female Rattus norvegicus over eight weeks of endurance exercise training. The resulting data compendium encompasses 9,466 assays across 19 tissues, 25 molecular platforms and 4 training time points. Thousands of shared and tissue-specific molecular alterations were identified, with sex differences found in multiple tissues. Temporal multi-omic and multi-tissue analyses revealed expansive biological insights into the adaptive responses to endurance training, including widespread regulation of immune, metabolic, stress response and mitochondrial pathways. Many changes were relevant to human health, including non-alcoholic fatty liver disease, inflammatory bowel disease, cardiovascular health and tissue injury and recovery. The data and analyses presented in this study will serve as valuable resources for understanding and exploring the multi-tissue molecular effects of endurance training and are provided in a public repository ( https://motrpac-data.org/ ).

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural

MeSH terms

  • Acetylation
  • Animals
  • Blood / immunology
  • Blood / metabolism
  • Cardiovascular Diseases / genetics
  • Cardiovascular Diseases / immunology
  • Cardiovascular Diseases / metabolism
  • Databases, Factual
  • Endurance Training*
  • Epigenome
  • Female
  • Humans
  • Inflammatory Bowel Diseases / genetics
  • Inflammatory Bowel Diseases / immunology
  • Inflammatory Bowel Diseases / metabolism
  • Internet
  • Lipidomics
  • Male
  • Metabolome
  • Mitochondria / metabolism
  • Multiomics*
  • Non-alcoholic Fatty Liver Disease / genetics
  • Non-alcoholic Fatty Liver Disease / immunology
  • Non-alcoholic Fatty Liver Disease / metabolism
  • Organ Specificity / genetics
  • Organ Specificity / immunology
  • Organ Specificity / physiology
  • Phosphorylation
  • Physical Conditioning, Animal* / physiology
  • Physical Endurance* / genetics
  • Physical Endurance* / physiology
  • Proteome / metabolism
  • Proteomics
  • Rats
  • Time Factors
  • Transcriptome / genetics
  • Ubiquitination
  • Wounds and Injuries / genetics
  • Wounds and Injuries / immunology
  • Wounds and Injuries / metabolism

Substances

  • Proteome