Nanopore sequencing from formalin-fixed paraffin-embedded specimens for copy-number profiling and methylation-based CNS tumor classification

Ann-Kristin Afflerbach; Anne Albers; Anton Appelt; Leonille Schweizer; Werner Paulus; Michael Bockmayr; Ulrich Schüller; Christian Thomas

doi:10.1007/s00401-024-02731-z

Nanopore sequencing from formalin-fixed paraffin-embedded specimens for copy-number profiling and methylation-based CNS tumor classification

Acta Neuropathol. 2024 Apr 20;147(1):74. doi: 10.1007/s00401-024-02731-z.

Authors

Ann-Kristin Afflerbach^{1

2

3}, Anne Albers⁴, Anton Appelt^{1

2}, Leonille Schweizer^{5

6

7}, Werner Paulus⁴, Michael Bockmayr^{1

2

8}, Ulrich Schüller^#^{1

2

9}, Christian Thomas^#¹⁰

Affiliations

¹ Department of Pediatric Hematology and Oncology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
² Research Institute Children's Cancer Center Hamburg, Hamburg, Germany.
³ Institute for Tumor Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
⁴ Institute of Neuropathology, University Hospital Münster, Pottkamp 2, 48149, Münster, Germany.
⁵ Institute of Neurology (Edinger Institute), University Hospital Frankfurt, Goethe University, Frankfurt Am Main, Germany.
⁶ German Cancer Research Center (DKFZ), German Cancer Consortium (DKTK), Partner Site Frankfurt/Mainz, Heidelberg, Germany.
⁷ Frankfurt Cancer Institute (FCI), Frankfurt Am Main, Germany.
⁸ bAIome - Center for Biomedical AI, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
⁹ Institute of Neuropathology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
¹⁰ Institute of Neuropathology, University Hospital Münster, Pottkamp 2, 48149, Münster, Germany. christian.thomas@ukmuenster.de.

^# Contributed equally.

No abstract available

Keywords: CNS tumors; Formalin-fixed paraffin-embedded; Nanopore sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Formaldehyde
Humans
Methylation
Nanopore Sequencing*
Neoplasms*
Paraffin Embedding
Tissue Fixation

Substances

Formaldehyde