A randomised controlled trial of Pre-Operative Oncotype DX testing in early-stage breast cancer (PRE-DX study) - Study protocol

PLoS One. 2024 Mar 15;19(3):e0300339. doi: 10.1371/journal.pone.0300339. eCollection 2024.

Abstract

Background: The Oncotype DX® Breast Recurrence Score assay can guide recommendations made to patients with oestrogen receptor positive (ER+), human epidermal growth factor receptor 2 negative (HER2-) breast cancer regarding post-surgery adjuvant therapy. Standard practice is to order the test in the post-operative setting on a specimen from the excised invasive carcinoma. However, it has been shown to be technically possible to perform the test on the diagnostic core biopsy. By testing the diagnostic core biopsy in the pre-operative setting, the wait for excised invasive carcinoma Recurrence Score results could be reduced allowing patients to be more accurately counselled regarding their treatment pathway sooner with any adjuvant treatment recommendations expedited. This would allow for more efficient streaming of follow up appointments. The aim of this study is to compare the impact on the patient treatment pathway of performing the Oncotype DX® test on the diagnostic core biopsy pre-operatively (intervention) as opposed to the excised invasive carcinoma (control).

Methods and analysis: This parallel group randomised controlled trial aims to recruit 330 newly diagnosed patients with grade 2 or grade 3, ER+, HER2-, invasive intermediate risk early-stage breast cancer. Participants will be randomised 2:1 to the preoperative testing of the diagnostic core biopsy compared to the post-operative testing of the excision specimen. The primary endpoint is number of clinical touchpoints between treating team and patient from initial approach until offer and prescription of the first adjuvant treatment. Secondary endpoints include time from diagnosis to offer and prescription of the first adjuvant treatment, patient-reported anxiety scores and health cost impact analysis collected at baseline, following the post-operative clinic and following the offer of adjuvant treatment, and number of alterations in treatment sequence from original planned surgical treatment to neoadjuvant therapy.

Trial registration: The study was registered on ISRCTN (ISRCTN14337451) on the 16th August 2022.

Publication types

  • Clinical Trial Protocol

MeSH terms

  • Adjuvants, Immunologic / therapeutic use
  • Breast Neoplasms* / diagnosis
  • Breast Neoplasms* / genetics
  • Breast Neoplasms* / surgery
  • Carcinoma* / drug therapy
  • Chemotherapy, Adjuvant / methods
  • Female
  • Gene Expression Profiling / methods
  • Humans
  • Neoadjuvant Therapy
  • Neoplasm Recurrence, Local / pathology
  • Randomized Controlled Trials as Topic
  • Receptors, Estrogen / metabolism

Substances

  • Receptors, Estrogen
  • Adjuvants, Immunologic

Grants and funding

The study is funded by a grant from Exact Sciences as an Investigator Initiated Trial. The funders had and will not have a role in study design, data collection and analysis, decision to publish, or preparation of the manuscript