The effectiveness of acellular nerve allografts compared to autografts in animal models: A systematic review and meta-analysis

PLoS One. 2024 Jan 31;19(1):e0279324. doi: 10.1371/journal.pone.0279324. eCollection 2024.

Abstract

Background: Treatment of nerve injuries proves to be a worldwide clinical challenge. Acellular nerve allografts are suggested to be a promising alternative for bridging a nerve gap to the current gold standard, an autologous nerve graft.

Objective: To systematically review the efficacy of the acellular nerve allograft, its difference from the gold standard (the nerve autograft) and to discuss its possible indications.

Material and methods: PubMed, Embase and Web of Science were systematically searched until the 4th of January 2022. Original peer reviewed paper that presented 1) distinctive data; 2) a clear comparison between not immunologically processed acellular allografts and autologous nerve transfers; 3) was performed in laboratory animals of all species and sex. Meta analyses and subgroup analyses (for graft length and species) were conducted for muscle weight, sciatic function index, ankle angle, nerve conduction velocity, axon count diameter, tetanic contraction and amplitude using a Random effects model. Subgroup analyses were conducted on graft length and species.

Results: Fifty articles were included in this review and all were included in the meta-analyses. An acellular allograft resulted in a significantly lower muscle weight, sciatic function index, ankle angle, nerve conduction velocity, axon count and smaller diameter, tetanic contraction compared to an autologous nerve graft. No difference was found in amplitude between acellular allografts and autologous nerve transfers. Post hoc subgroup analyses of graft length showed a significant reduced muscle weight in long grafts versus small and medium length grafts. All included studies showed a large variance in methodological design.

Conclusion: Our review shows that the included studies, investigating the use of acellular allografts, showed a large variance in methodological design and are as a consequence difficult to compare. Nevertheless, our results indicate that treating a nerve gap with an allograft results in an inferior nerve recovery compared to an autograft in seven out of eight outcomes assessed in experimental animals. In addition, based on our preliminary post hoc subgroup analyses we suggest that when an allograft is being used an allograft in short and medium (0-1cm, > 1-2cm) nerve gaps is preferred over an allograft in long (> 2cm) nerve gaps.

Publication types

  • Meta-Analysis
  • Systematic Review

MeSH terms

  • Allografts / transplantation
  • Animals
  • Autografts / transplantation
  • Nerve Regeneration* / physiology
  • Sciatic Nerve* / injuries
  • Transplantation, Autologous / methods
  • Transplantation, Homologous / methods

Grants and funding

The authors will receive an award from ZonMw upon publication. Partially to make open access publication possible. ZonMw is an independent institute which has no benefits from the publicated data of this article. Therefore, the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Initials receiving author: T. De Jong Grant number: 114024160 Name funder: ZonMw URL:https://www.zonmw.nl/nl/ The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.