Characterization of Two-Component System gene (TCS) in melatonin-treated common bean under salt and drought stress

Ayse Gul Kasapoglu; Emre Ilhan; Murat Aydin; Esma Yigider; Behcet Inal; Ilker Buyuk; Mahmut Sinan Taspinar; Abdulkadir Ciltas; Guleray Agar

doi:10.1007/s12298-023-01406-5

Characterization of Two-Component System gene (TCS) in melatonin-treated common bean under salt and drought stress

Physiol Mol Biol Plants. 2023 Nov;29(11):1733-1754. doi: 10.1007/s12298-023-01406-5. Epub 2023 Dec 23.

Authors

Ayse Gul Kasapoglu¹, Emre Ilhan¹, Murat Aydin², Esma Yigider², Behcet Inal³, Ilker Buyuk⁴, Mahmut Sinan Taspinar², Abdulkadir Ciltas², Guleray Agar⁵

Affiliations

¹ Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, 25050 Erzurum, Turkey.
² Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, 25050 Erzurum, Turkey.
³ Department of Agricultural Biotechnology, Faculty of Agriculture, Siirt University, 56100 Siirt, Turkey.
⁴ Department of Biology, Faculty of Science, Ankara University, 06100 Ankara, Turkey.
⁵ Department of Biology, Faculty of Science, Ataturk University, 25050 Erzurum, Turkey.

Abstract

The two-component system (TCS) generally consists of three elements, namely the histidine kinase (HK), response regulator (RR), and histidine phosphotransfer (HP) gene families. This study aimed to assess the expression of TCS genes in P. vulgaris leaf tissue under salt and drought stress and perform a genome-wide analysis of TCS gene family members using bioinformatics methods. This study identified 67 PvTCS genes, including 10 PvHP, 38 PvRR, and 19 PvHK, in the bean genome. PvHK2 had the maximum number of amino acids with 1261, whilst PvHP8 had the lowest number with 87. In addition, their theoretical isoelectric points were between 4.56 (PvHP8) and 9.15 (PvPRR10). The majority of PvTCS genes are unstable. Phylogenetic analysis of TCS genes in A. thaliana, G. max, and bean found that PvTCS genes had close phylogenetic relationships with the genes of other plants. Segmental and tandem duplicate gene pairs were detected among the TCS genes and TCS genes have been subjected to purifying selection pressure in the evolutionary process. Furthermore, the TCS gene family, which has an important role in abiotic stress and hormonal responses in plants, was characterized for the first time in beans, and its expression of TCS genes in bean leaves under salt and drought stress was established using RNAseq and qRT-PCR analyses. The findings of this study will aid future functional and genomic studies by providing essential information about the members of the TCS gene family in beans.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-023-01406-5.

Keywords: Expression profile; Histidine; Melatonin; Phaseolus vulgaris; Two component system (TCS).

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