Characterization of immune populations in the tumor microenvironment of diffuse midline glioma orthotopic mouse models by flow cytometry

Iker Ausejo-Mauleon; Sara Labiano; Marta M Alonso

doi:10.1016/j.xpro.2023.102803

Characterization of immune populations in the tumor microenvironment of diffuse midline glioma orthotopic mouse models by flow cytometry

STAR Protoc. 2024 Mar 15;5(1):102803. doi: 10.1016/j.xpro.2023.102803. Epub 2023 Dec 29.

Authors

Iker Ausejo-Mauleon¹, Sara Labiano², Marta M Alonso³

Affiliations

¹ Health Research Institute of Navarra (IdiSNA), Pamplona, Spain; Solid Tumor Program, CIMA-Universidad de Navarra, Pamplona, Spain; Department of Pediatrics, Clínica Universidad de Navarra, Pamplona, Spain.
² Health Research Institute of Navarra (IdiSNA), Pamplona, Spain; Solid Tumor Program, CIMA-Universidad de Navarra, Pamplona, Spain; Department of Pediatrics, Clínica Universidad de Navarra, Pamplona, Spain. Electronic address: slalminana@unav.es.
³ Health Research Institute of Navarra (IdiSNA), Pamplona, Spain; Solid Tumor Program, CIMA-Universidad de Navarra, Pamplona, Spain; Department of Pediatrics, Clínica Universidad de Navarra, Pamplona, Spain. Electronic address: mmalonso@unav.es.

Abstract

The immune response is a fundamental process in the treatment of solid tumors. Here, we present a protocol for implanting diffuse midline glioma cells in the brain of immune-competent mice and characterizing the different immune populations in the tumor microenvironment in a flow cytometry panel. We describe steps for processing of brain tumors, isolating the immune cells, and subsequent staining with antibodies for flow cytometry. We then detail procedures for implementing the gating strategy. For complete details on the use and execution of this protocol, please refer to Ausejo-Mauleon et al.¹.

Keywords: Cancer; Flow Cytometry; Immunology.

MeSH terms

Animals
Antibodies
Brain
Disease Models, Animal
Flow Cytometry
Glioma*
Mice
Tumor Microenvironment*

Substances

Antibodies