Native and Ion Mobility Mass Spectrometry Characterization of Alpha 1 Antitrypsin Variants and Oligomers

Sarah Vickers; James Irving; David A Lomas; Konstantinos Thalassinos

doi:10.1007/978-1-0716-3605-3_5

Native and Ion Mobility Mass Spectrometry Characterization of Alpha 1 Antitrypsin Variants and Oligomers

Methods Mol Biol. 2024:2750:41-55. doi: 10.1007/978-1-0716-3605-3_5.

Authors

Sarah Vickers^{1

2}, James Irving^{2

3}, David A Lomas³, Konstantinos Thalassinos^{4

5}

Affiliations

¹ Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, UK.
² Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, UK.
³ UCL Respiratory, University College London, London, UK.
⁴ Institute of Structural and Molecular Biology, Division of Biosciences, University College London, London, UK. k.thalassinos@ucl.ac.uk.
⁵ Institute of Structural and Molecular Biology, Birkbeck College, University of London, London, UK. k.thalassinos@ucl.ac.uk.

PMID: 38108966
DOI: 10.1007/978-1-0716-3605-3_5

Abstract

In this chapter, we describe a method for analyzing both recombinant and plasma-derived alpha 1 antitrypsin and its oligomers by means of native ion mobility mass spectrometry. Our experimental workflow can be applied to other variants of alpha 1 antitrypsin and its oligomers as well as being used to probe their interactions with small molecules in the gas phase.

Keywords: Alpha 1 antitrypsin; Collisional activation; Conformational diseases; Ion mobility spectrometry; Mass spectrometry; Proteins; Serpins.

MeSH terms

Ion Mobility Spectrometry*
Mass Spectrometry
Plasma
Workflow
alpha 1-Antitrypsin* / genetics

Substances

alpha 1-Antitrypsin

Grants and funding

MR/V034243/1/MRC_/Medical Research Council/United Kingdom