Treadmill exercise with nanoselenium supplementation affects the expression of Irisin/FNDC5 and semaphorin 3A in rats exposed to cigarette smoke extract

Ehsan Arabzadeh; Hossein Shirvani; Mohammad Reza Masjedi; Mostafa Ghanei; Martin Hofmeister; Fatemeh Rostamkhani

doi:10.1007/s13205-023-03849-9

Treadmill exercise with nanoselenium supplementation affects the expression of Irisin/FNDC5 and semaphorin 3A in rats exposed to cigarette smoke extract

3 Biotech. 2024 Jan;14(1):4. doi: 10.1007/s13205-023-03849-9. Epub 2023 Dec 4.

Authors

Ehsan Arabzadeh¹, Hossein Shirvani¹, Mohammad Reza Masjedi², Mostafa Ghanei³, Martin Hofmeister⁴, Fatemeh Rostamkhani⁵

Affiliations

¹ Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
² Tobacco Control Research Center (TCRC), Iranian Anti-Tobacco Association, Tehran, Iran.
³ Chemical Injuries Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
⁴ Department of Food and Nutrition, Consumer Centre of the German Federal State of Bavaria, Munich, Germany.
⁵ Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran.

PMID: 38058362
PMCID: PMC10695908 (available on 2025-01-01)
DOI: 10.1007/s13205-023-03849-9

Abstract

In the current study, we investigated the impacts of 6 weeks of aerobic interval training (AIT) with selenium nanoparticles (SeNPs) on muscle, serum, and lung irisin (FNDC5) and Sema3A in rats exposed to cigarette smoke extract (CSE). To this end, 49 male Wistar rats (8 weeks old) were divided into seven groups: control, SeNPs (2.5 mg/kg b.w by oral gavage, 3 days/week, 6 weeks), AIT (49 min/day, 5 days/week for 6 weeks, interval), SeNPs + AIT, CSE (150 µL by IP injection, 1 day/week for 6 weeks), CSE + AIT, and CSE + SeNPs + AIT. The CSE group showed a significant reduction in irisin and Sema3A serum levels, as well as a decrease in FNDC5 and Sema3A gene expression in lung tissue (p < 0.05). A combined treatment (AIT with SeNPs) significantly increased the serum level and the expression of muscle and lung irisin (FNDC5) and Sema3A in CSE received groups (p < 0.05). There was a positive and significant correlation between muscle FNDC5 and lung FNDC5 in the CSE + SeNPs + AIT group (r = 0.92, p = 0.025). In addition, there was a positive and significant correlation between serum Sema3A and lung Sema3A of CSE + SeNPs + AIT group (r = 0.97, p = 0.004). Seemingly, performing aerobic exercises with the antioxidant and anti-inflammatory supplement nano-selenium in the model of lung damage (similar to COPD) can boost myokine irisin and Sema3A, especially in serum and lung tissue. These results displayed the paracrine/endocrine regulatory function of these myokines on other tissues. In other words, these interventions emphasized the creation of crosstalk between skeletal muscles and damaged lung, focusing on its recovery; however, further research is needed.

Keywords: Aerobic exercise; Chronic obstructive pulmonary disease; Cigarette smoking; Selenium nanoparticles; Skeletal muscle.

© King Abdulaziz City for Science and Technology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.