Measuring effect of mutations & conditions on microbial respiratory rates

J Microbiol Methods. 2024 Jan:216:106864. doi: 10.1016/j.mimet.2023.106864. Epub 2023 Nov 27.

Abstract

Cellular respiration is central to a wide range of cellular processes. In microorganisms, the effect of a mutation or an environmental condition on the rate of respiration is usually determined by measuring oxygen consumption in the media. We describe this method and discuss caveats and controls for the method.

Keywords: BOD; Dissolved oxygen; Mutant; Respiration rate; Yeast.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Mutation
  • Oxygen Consumption
  • Oxygen*
  • Respiratory Rate*

Substances

  • Oxygen