Progenitor Cell Sources for 3D Bioprinting of Lymphatic Vessels and Potential Clinical Application

Tissue Eng Part A. 2024 Jul;30(13-14):353-366. doi: 10.1089/ten.TEA.2023.0204. Epub 2023 Dec 22.

Abstract

The lymphatic system maintains tissue fluid homeostasis and it is involved in the transport of nutrients and immunosurveillance. It also plays a pivotal role in both pathological and regenerative processes. Lymphatic development in the embryo occurs by polarization and proliferation of lymphatic endothelial cells from the lymph sacs, that is, lymphangiogenesis. Alternatively, lymphvasculogenesis further contributes to the formation of lymphatic vessels. In adult tissues, lymphatic formation rarely occurs under physiological conditions, being restricted to pathological processes. In lymphvasculogenesis, progenitor cells seem to be a source of lymphatic vessels. Indeed, mesenchymal stem cells, adipose stem cells, endothelial progenitor cells, and colony-forming endothelial cells are able to promote lymphatic regeneration by different mechanisms, such as direct differentiation and paracrine effects. In this review, we summarize what is known on the diverse stem/progenitor cell niches available for the lymphatic system, emphasizing the potential that these cells hold for lymphatic tissue engineering through 3D bioprinting and their translation to clinical application.

Keywords: adipose tissue; bone marrow; dermis; lymphatic endothelial cells; lymphatic endothelial progenitor cells.

Publication types

  • Review

MeSH terms

  • Animals
  • Bioprinting* / methods
  • Humans
  • Lymphangiogenesis
  • Lymphatic Vessels* / cytology
  • Printing, Three-Dimensional*
  • Stem Cells* / cytology
  • Stem Cells* / metabolism
  • Tissue Engineering / methods