GWAS of lipids in Greenlanders finds association signals shared with Europeans and reveals an independent PCSK9 association signal

Eur J Hum Genet. 2024 Feb;32(2):215-223. doi: 10.1038/s41431-023-01485-8. Epub 2023 Oct 30.

Abstract

Perturbation of lipid homoeostasis is a major risk factor for cardiovascular disease (CVD), the leading cause of death worldwide. We aimed to identify genetic variants affecting lipid levels, and thereby risk of CVD, in Greenlanders. Genome-wide association studies (GWAS) of six blood lipids, triglycerides, LDL-cholesterol, HDL-cholesterol, total cholesterol, as well as apolipoproteins A1 and B, were performed in up to 4473 Greenlanders. For genome-wide significant variants, we also tested for associations with additional traits, including CVD events. We identified 11 genome-wide significant loci associated with lipid traits. Most of these loci were already known in Europeans, however, we found a potential causal variant near PCSK9 (rs12117661), which was independent of the known PCSK9 loss-of-function variant (rs11491147). rs12117661 was associated with lower LDL-cholesterol (βSD(SE) = -0.22 (0.03), p = 6.5 × 10-12) and total cholesterol (-0.17 (0.03), p = 1.1 × 10-8) in the Greenlandic study population. Similar associations were observed in Europeans from the UK Biobank, where the variant was also associated with a lower risk of CVD outcomes. Moreover, rs12117661 was a top eQTL for PCSK9 across tissues in European data from the GTEx portal, and was located in a predicted regulatory element, supporting a possible causal impact on PCSK9 expression. Combined, the 11 GWAS signals explained up to 16.3% of the variance of the lipid traits. This suggests that the genetic architecture of lipid levels in Greenlanders is different from Europeans, with fewer variants explaining the variance.

MeSH terms

  • Cardiovascular Diseases* / genetics
  • Cholesterol, HDL
  • Cholesterol, LDL / genetics
  • Cholesterol, LDL / metabolism
  • Genome-Wide Association Study*
  • Greenland
  • Humans
  • Lipids / genetics
  • Polymorphism, Single Nucleotide
  • Proprotein Convertase 9 / genetics
  • Triglycerides / genetics

Substances

  • PCSK9 protein, human
  • Proprotein Convertase 9
  • Triglycerides
  • Lipids
  • Cholesterol, HDL
  • Cholesterol, LDL