Human ApoE2 protects mice against Plasmodium berghei ANKA experimental cerebral malaria

mBio. 2023 Dec 19;14(6):e0234623. doi: 10.1128/mbio.02346-23. Epub 2023 Oct 24.

Abstract

Cerebral malaria (CM) is the deadliest complication of malaria infection with an estimated 15%-25% mortality. Even with timely and effective treatment with antimalarial drugs such as quinine and artemisinin derivatives, survivors of CM may suffer long-term cognitive and neurological impairment. Here, we show that human apolipoprotein E variant 2 (hApoE2) protects mice from experimental CM (ECM) via suppression of CD8+ T cell activation and infiltration to the brain, enhanced cholesterol metabolism, and increased IFN-γ production, leading to reduced endothelial cell apoptosis, BBB disruption, and ECM symptoms. Our results suggest that hApoE can be an important factor for risk assessment and treatment of CM in humans.

Keywords: apolipoprotein E; blood-brain barrier; immune response; lipid metabolism; transcriptome.

MeSH terms

  • Animals
  • Apolipoprotein E2 / genetics
  • Apoptosis
  • Blood-Brain Barrier / parasitology
  • Brain / metabolism
  • Brain / parasitology
  • Brain / pathology
  • CD8-Positive T-Lymphocytes / immunology
  • Disease Models, Animal*
  • Endothelial Cells / parasitology
  • Humans
  • Interferon-gamma / genetics
  • Interferon-gamma / immunology
  • Interferon-gamma / metabolism
  • Malaria, Cerebral* / parasitology
  • Mice
  • Mice, Inbred C57BL
  • Plasmodium berghei* / genetics

Substances

  • Apolipoprotein E2
  • Interferon-gamma