According to ambivalent sexism theory (Glick & Fiske, 1996), the coexistence of gendered power differences and mutual interdependence creates two apparently opposing but complementary sexist ideologies: hostile sexism (HS; viewing women as manipulative competitors who seek to gain power over men) coincides with benevolent sexism (BS; a chivalrous view of women as pure and moral, yet weak and passive, deserving men's protection and admiration, as long as they conform). The research on these ideologies employs the Ambivalent Sexism Inventory, used extensively in psychology and allied disciplines, often to understand the roles sexist attitudes play in reinforcing gender inequality. Following contemporary guidelines, this systematic review utilizes a principled approach to synthesize the multidisciplinary empirical literature on ambivalent sexism. After screening 1,870 potentially relevant articles and fully reviewing 654 eligible articles, five main domains emerge in ambivalent sexism research (social ideologies, violence, workplace, stereotypes, intimate relationships). The accumulating evidence across domains offers bottom-up empirical support for ambivalent sexism as a coordinated system to maintain control over women (and sometimes men). Hostile sexism acts through the direct and diverse paths of envious/resentful prejudices, being more sensitive to power and sexuality cues; Benevolent sexism acts through prejudices related to interdependence (primarily gender-based paternalism and gender-role differentiation), enforcing traditional gender relations and being more sensitive to role-related cues. Discussion points to common methodological limitations, suggests guidelines, and finds future avenues for ambivalent sexism research. (PsycInfo Database Record (c) 2023 APA, all rights reserved).