Relative biochemical and physiological docking of cucumber varieties for supporting innate immunity against Podosphaera xanthii

Maryam M Alomran; Ali Noman; Muhammad Aqeel; Noreen Khalid; Muhammad Faisal Maqsood; Noreen Akhter; Muhammad Arshad; Fatmah M Alqahtani; Fahad Mohammed Alzuaibr; Mohamed Hashem; Talaat Habeeb; Omar Mahmoud Al-Zoubi; Modhi O Alotaibi

doi:10.1016/j.micpath.2023.106359

Relative biochemical and physiological docking of cucumber varieties for supporting innate immunity against Podosphaera xanthii

Microb Pathog. 2023 Nov:184:106359. doi: 10.1016/j.micpath.2023.106359. Epub 2023 Sep 15.

Authors

Affiliations

¹ Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia.
² Department of Botany, Government College University, Faisalabad, Pakistan.
³ State Key Laboratory of Herbage Improvement and Grassland Agroecosystems (SKLHIGA), College of Ecology, Lanzhou University, Lanzhou, 730000, Gansu, PR China.
⁴ Department of Botany, Government College Women University Sialkot, Pakistan.
⁵ Department of Botany, The Islamia University of Bahawalpur, Pakistan.
⁶ Department of Botany, Government College Women University Faisalabad, Pakistan.
⁷ Department of Biology, Faculty of Science, King Khalid University, Abha, 61413, Saudi Arabia.
⁸ Department of Biology, Faculty of Science, University of Tabuk, Tabuk, 47713, Saudi Arabia.
⁹ Biology Department, Faculty of Science Yanbu, Taibah University, Yanbu El-Bahr, 46423, Saudi Arabia.
¹⁰ Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia. Electronic address: mouotabe@pnu.edu.sa.

PMID: 37716624
DOI: 10.1016/j.micpath.2023.106359

Abstract

Powdery mildew in cucumber is caused by the Podosphaera xanthii. No strategy for improving disease resistance can be successful in the absence of thorough insights into the physiological and biochemical responses of cucumber plants to powdery mildew. Therefore, a field experiment was executed to evaluate five commercial cucumber varieties (V₁: Dynasty, V₂: Long green, V₃:Desi Kheera, V₄:Thamin II, V₅:Cucumber 363) for their inherent immunity to powdery mildew. Upon inoculating cucumber plants with Podosphaera xanthii, we noted differential responses among the varieties. Compared to other varieties, V₁ and V₂ showed higher values (P ≤ 0.05) for chlorophyll-a under control and pathogen-attacked plants respectively. The minimum value of anthocyanin content (-53.73%) was recorded in V₃ as compared to other varieties post pathogen infection. All pathogen-infected cucumber varieties showed a considerable (P ≤ 0.05) loss in flavonoid content except V₂. The maximum destruction for Phenolics under powdery mildew (179%) were recorded in V₄, whereas V₁ exhibited maximum phenolic content under control conditions. In pathogen-infected plants, the minimum AsA was recorded in V₅ as compared to all other varieties. Pathogen invasion impacted significantly (P ≤ 0.05) the activity of superoxide dismutase (SOD). Besides, cucumber plants after pathogen inoculation resulted in a considerable (P ≤ 0.05) increase of peroxidase (POD) activity in V₁ (5.02%), V₂ (7.5%), and V₃ (11%) in contrast to V₄. Our results confirmed that cucumber varieties perform differently, which was brought on by distinct metabolic and physiological modifications that have an impact on growth and development. The changes in different attributes were correlated with cucumber resistance against powdery mildew. The results would help us fully harness the potential of these varieties to trigger disease management initiatives and defense responses.

Keywords: Biotic stress; Disease resistance; Inherent immunity; Inoculation; Pathogen invasion; Powdery mildew.

MeSH terms

Ascomycota* / physiology
Cucumis sativus*
Disease Resistance

Supplementary concepts

Podosphaera xanthii