POU2F1/DNMT3a Pathway Participates in Neuropathic Pain by Hypermethylation-Mediated LRFN4 Downregulation Following Oxaliplatin Treatment

Neurochem Res. 2023 Dec;48(12):3652-3664. doi: 10.1007/s11064-023-04011-w. Epub 2023 Aug 18.

Abstract

Evidence demonstrates that DNA methylation is associated with the occurrence and development of various neurological diseases. However, the potential target genes undergoing DNA methylation, as well as their involvement in the chemotherapy drug oxaliplatin-induced neuropathic pain, are still unclear. Here, Lrfn4, which showed hypermethylation in the promoter regions, was screened from the SRA methylation database (PRJNA587622) following oxaliplatin treatment. MeDIP and qPCR assays identified that oxaliplatin treatment increased the methylation in Lrfn4 promoter region and decreased the expression of LRFN4 in the spinal dorsal horn. The assays with gain and loss of LRFN4 function demonstrated that LRFN4 downregulation in spinal dorsal horn contributed to the oxaliplatin-induced mechanical allodynia and cold hyperalgesia. Moreover, oxaliplatin treatment increased the DNA methyltransferases DNMT3a expression and the interaction between DNMT3a and Lrfn4 promoter, while inhibition of DNMT3a prevented the downregulation of LRFN4a induced by oxaliplatin. We also observed that the transcriptional factor POU2F1 can bind to the predicted sites in DNMT3a promoter region, oxaliplatin treatment upregulated the expression of transcriptional factor POU2F1 in dorsal horn neurons. Intrathecal injection of POU2F1 siRNA prevented the DNMT3a upregulation and the LRFN4 downregulation induced by oxaliplatin. Additionally, intrathecal injection of DNMT3a siRNA or POU2F1 siRNA alleviated the mechanical allodynia induced by oxaliplatin. These findings suggested that transcription factor POU2F1 upregulated the expression of DNMT3a, which subsequently decreased LRFN4 expression through hypermethylation modification in spinal dorsal horn, thereby mediating neuropathic pain following oxaliplatin treatment.

Keywords: Methylation; Neuropathic pain; Oxaliplatin; Spinal dorsal horn; Transcription factors.

MeSH terms

  • Animals
  • DNA Methylation*
  • Down-Regulation
  • Hyperalgesia / metabolism
  • Membrane Glycoproteins / metabolism
  • Nerve Tissue Proteins / metabolism
  • Neuralgia* / chemically induced
  • Neuralgia* / drug therapy
  • Neuralgia* / metabolism
  • Octamer Transcription Factor-1 / metabolism
  • Oxaliplatin / adverse effects
  • RNA, Small Interfering / therapeutic use
  • Rats
  • Spinal Cord Dorsal Horn / metabolism

Substances

  • DNMT3A protein, rat
  • Membrane Glycoproteins
  • Nerve Tissue Proteins
  • Octamer Transcription Factor-1
  • Oxaliplatin
  • Pou2f1 protein, rat
  • RNA, Small Interfering
  • Lrfn4 protein, rat