Overexpression of OsPIN9 Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice

Qiqi Ouyang; Yanwen Zhang; Xiaoyi Yang; Chong Yang; Dianyun Hou; Hao Liu; Huawei Xu

doi:10.3390/plants12152809

Overexpression of OsPIN9 Impairs Chilling Tolerance via Disturbing ROS Homeostasis in Rice

Plants (Basel). 2023 Jul 28;12(15):2809. doi: 10.3390/plants12152809.

Authors

Qiqi Ouyang¹, Yanwen Zhang¹, Xiaoyi Yang¹, Chong Yang¹, Dianyun Hou¹, Hao Liu¹, Huawei Xu¹

Affiliation

¹ College of Agriculture, Henan University of Science and Technology, Luoyang 471000, China.

Abstract

The auxin efflux transporter PIN-FORMED (PIN) family is one of the major protein families that facilitates polar auxin transport in plants. Here, we report that overexpression of OsPIN9 leads to altered plant architecture and chilling tolerance in rice. The expression profile analysis indicated that OsPIN9 was gradually suppressed by chilling stress. The shoot height and adventitious root number of OsPIN9-overexpressing (OE) plants were significantly reduced at the seedling stage. The roots of OE plants were more tolerant to N-1-naphthylphthalamic acid (NPA) treatment than WT plants, indicating the disturbance of auxin homeostasis in OE lines. The chilling tolerance assay showed that the survival rate of OE plants was markedly lower than that of wild-type (WT) plants. Consistently, more dead cells, increased electrolyte leakage, and increased malondialdehyde (MDA) content were observed in OE plants compared to those in WT plants under chilling conditions. Notably, OE plants accumulated more hydrogen peroxide (H₂O₂) and less superoxide anion radicals (O2-) than WT plants under chilling conditions. In contrast, catalase (CAT) and superoxide dismutase (SOD) activities in OE lines decreased significantly compared to those in WT plants at the early chilling stage, implying that the impaired chilling tolerance of transgenic plants is probably attributed to the sharp induction of H₂O₂ and the delayed induction of antioxidant enzyme activities at this stage. In addition, several OsRboh genes, which play a crucial role in ROS production under abiotic stress, showed an obvious increase after chilling stress in OE plants compared to that in WT plants, which probably at least in part contributes to the production of ROS under chilling stress in OE plants. Together, our results reveal that OsPIN9 plays a vital role in regulating plant architecture and, more importantly, is involved in regulating rice chilling tolerance by influencing auxin and ROS homeostasis.

Keywords: OsPIN9; ROS homeostasis; antioxidant enzymes; chilling tolerance; polar auxin transport; rice (Oryza sativa L.).

Grants and funding

This work was funded by the National Natural Science Foundation of China (grant number 3110019) and the Natural Science Foundation of Henan Province (grant number 182300410012).