Knockdown of SDC-1 Gene Alleviates the Metabolic Pathway for the Development of MODS

Mol Biotechnol. 2024 Aug;66(8):1961-1969. doi: 10.1007/s12033-023-00809-9. Epub 2023 Jul 29.

Abstract

This study aims to reveal the metabolic differences between SDC-1 knockout mice and wild-type mice and the metabolic differences caused by shock in SDC-1 knockout mice by integrating transcriptomics and metabolomics. A total of 1009 differential metabolites were differentially expressed based on untargeted metabolomics and high-resolution mass spectrometry detection techniques. According to Kyoto Encyclopedia of Genes and Genomes enrichment, SDC-1 knockout significantly altered fat digestion and absorption, GnRH signaling pathway, fructose and mannose metabolism, and some other amino-related metabolic pathways and significantly modulated positively regulated longevity regulatory pathways, longevity regulatory pathways-worm, nicotinamide and niacinamide metabolism, and vitamin digestion and absorption pathways after its shock. Our findings indicate that SDC-1 knockout may have potential therapeutic effects in hemorrhagic shock by increasing nicotinamide metabolism.

Keywords: MODS; Nicotinamide; Nicotinamide metabolism; Syndecan-1.

MeSH terms

  • Animals
  • Gene Knockdown Techniques
  • Male
  • Metabolic Networks and Pathways* / genetics
  • Metabolomics / methods
  • Mice
  • Mice, Knockout*
  • Niacinamide / metabolism
  • Shock, Hemorrhagic / genetics
  • Shock, Hemorrhagic / metabolism
  • Shock, Hemorrhagic / therapy
  • Syndecan-1 / genetics
  • Syndecan-1 / metabolism

Substances

  • Syndecan-1
  • Niacinamide