Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/ β-Catenin Pathway

Yan Zhang; Zhuna Yan; Hui Wu; Xiao Yang; Ke Yang; Wencheng Song

doi:10.3390/biom13071073

Low-Temperature Plasma-Activated Medium Inhibits the Migration of Non-Small Cell Lung Cancer Cells via the Wnt/ β-Catenin Pathway

Biomolecules. 2023 Jul 4;13(7):1073. doi: 10.3390/biom13071073.

Authors

Yan Zhang^{1

2}, Zhuna Yan^{1

2}, Hui Wu², Xiao Yang², Ke Yang², Wencheng Song^{1

2

3

4}

Affiliations

¹ School of Medicine, Anhui University of Science and Technology, Huainan 232001, China.
² Anhui Institute of Optics and Fine Mechanics, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.
³ Hefei Cancer Hospital, Chinese Academy of Sciences, Hefei 230031, China.
⁴ Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions and School for Radiological and Interdisciplinary Sciences, Soochow University, Suzhou 215123, China.

Abstract

This study explored the molecular mechanism of the plasma activation medium (PAM) inhibiting the migration ability of NSCLC (non-small cell lung cancer) cells. The effect of PAM incubation on the cell viability of NSCLC was detected through a cell viability experiment. Transwell cells and microfluidic chips were used to investigate the effects of PAM on the migration capacity of NSCLC cells, and the latter was used for the first time to observe the changes in the migration capacity of cancer cells treated with PAM. Moreover, the molecular mechanisms of PAM affecting the migration ability of NSCLC cells were investigated through intracellular and extracellular ROS detection, mitochondrial membrane potential, and Western blot experiments. The results showed that after long-term treatment with PAM, the high level of ROS produced by PAM reduced the level of the mitochondrial membrane potential of cells and blocked the cell division cycle in the G2/M phase. At the same time, the EMT process was reversed by inhibiting the Wnt/β-catenin signaling pathway. These results suggested that the high ROS levels generated by the PAM treatment reversed the EMT process by inhibiting the WNT/β-catenin pathway in NSCLC cells and thus inhibited the migration of NSCLC cells. Therefore, these results provide good theoretical support for the clinical treatment of NSCLC with PAM.

Keywords: NSCLC; RONS; Wnt/β-catenin; migration; plasma-activated medium.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma, Non-Small-Cell Lung* / metabolism
Cell Line, Tumor
Cell Movement
Cell Proliferation
Humans
Lung Neoplasms* / drug therapy
Lung Neoplasms* / metabolism
Reactive Oxygen Species
Temperature
Wnt Signaling Pathway
beta Catenin / metabolism

Substances

beta Catenin
Reactive Oxygen Species

Grants and funding

This study was supported by the Natural Science Foundation of China (21876179), the National Key Research and Development Program (2022YFC2302703), and the HFIPS Director’s Fund (2023YZGH02).