Spatiotemporal proteomic atlas of multiple brain regions across early fetal to neonatal stages in cynomolgus monkey

Jingkuan Wei; Shaoxing Dai; Yaping Yan; Shulin Li; Pengpeng Yang; Ran Zhu; Tianzhuang Huang; Xi Li; Yanchao Duan; Zhengbo Wang; Weizhi Ji; Wei Si

doi:10.1038/s41467-023-39411-7

Spatiotemporal proteomic atlas of multiple brain regions across early fetal to neonatal stages in cynomolgus monkey

Nat Commun. 2023 Jul 3;14(1):3917. doi: 10.1038/s41467-023-39411-7.

Authors

Jingkuan Wei^#^{1

2}, Shaoxing Dai^#^{1

2}, Yaping Yan^#^{1

2}, Shulin Li¹, Pengpeng Yang¹, Ran Zhu¹, Tianzhuang Huang^{1

2}, Xi Li^{1

2}, Yanchao Duan^{1

2}, Zhengbo Wang^{3

4}, Weizhi Ji^{5

6

7}, Wei Si^{8

9

10}

Affiliations

¹ State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, 650500, Kunming, Yunnan, China.
² Yunnan Key Laboratory of Primate Biomedical Research, 650500, Kunming, Yunnan, China.
³ State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, 650500, Kunming, Yunnan, China. wangzb@lpbr.cn.
⁴ Yunnan Key Laboratory of Primate Biomedical Research, 650500, Kunming, Yunnan, China. wangzb@lpbr.cn.
⁵ State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, 650500, Kunming, Yunnan, China. wji@lpbr.cn.
⁶ Yunnan Key Laboratory of Primate Biomedical Research, 650500, Kunming, Yunnan, China. wji@lpbr.cn.
⁷ Chinese Primate Biomedical Research Alliance (CPBRA), 650500, Kunming, Yunnan, China. wji@lpbr.cn.
⁸ State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, 650500, Kunming, Yunnan, China. siw@lpbr.cn.
⁹ Yunnan Key Laboratory of Primate Biomedical Research, 650500, Kunming, Yunnan, China. siw@lpbr.cn.
¹⁰ Chinese Primate Biomedical Research Alliance (CPBRA), 650500, Kunming, Yunnan, China. siw@lpbr.cn.

^# Contributed equally.

Abstract

Fetal stages are critical periods for brain development. However, the protein molecular signature and dynamics of the human brain remain unclear due to sampling difficulty and ethical limitations. Non-human primates present similar developmental and neuropathological features to humans. This study constructed a spatiotemporal proteomic atlas of cynomolgus macaque brain development from early fetal to neonatal stages. Here we showed that (1) the variability across stages was greater than that among brain regions, and comparisons of cerebellum vs. cerebrum and cortical vs. subcortical regions revealed region-specific dynamics across early fetal to neonatal stages; (2) fluctuations in abundance of proteins associated with neural disease suggest the risk of nervous disorder at early fetal stages; (3) cross-species analysis (human, monkey, and mouse) and comparison between proteomic and transcriptomic data reveal the proteomic specificity and genes with mRNA/protein discrepancy. This study provides insight into fetal brain development in primates.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain* / metabolism
Cerebellum
Fetal Development
Macaca fascicularis
Proteomics*

Grants and funding

R21 AG050018/AG/NIA NIH HHS/United States