A black goat-derived novel genotype of Aichi virus C blurs the boundary between caprine and porcine kobuviruses

Mengkun Huang; Jinxian Gan; Ziqian Xu; Yuanyuan Guo; Zhangfu Chen; George F Gao; Hao Liang; William J Liu

doi:10.1016/j.virol.2023.06.014

A black goat-derived novel genotype of Aichi virus C blurs the boundary between caprine and porcine kobuviruses

Virology. 2023 Aug:585:215-221. doi: 10.1016/j.virol.2023.06.014. Epub 2023 Jun 24.

Authors

Mengkun Huang¹, Jinxian Gan¹, Ziqian Xu², Yuanyuan Guo³, Zhangfu Chen³, George F Gao⁴, Hao Liang⁵, William J Liu⁶

Affiliations

¹ Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, Guangxi Medical University, Nanning, 530000, China; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, 102206, China.
² NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, 102206, China.
³ NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, 102206, China; School of Public Health, Shandong University, Jinan, 250012, China.
⁴ Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, Guangxi Medical University, Nanning, 530000, China; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, 102206, China; School of Public Health, Shandong University, Jinan, 250012, China; CAS Key Laboratory of Pathogen Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing, 100101, China; Research Unit of Adaptive Evolution and Control of Emerging Viruses (2018RU009), Chinese Academy of Medical Sciences, Beijing, 102206, China. Electronic address: gaof@im.ac.cn.
⁵ Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, Guangxi Medical University, Nanning, 530000, China. Electronic address: lianghao@gxmu.edu.cn.
⁶ Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed By the Province and Ministry, Guangxi Medical University, Nanning, 530000, China; NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (China CDC), Beijing, 102206, China; Research Unit of Adaptive Evolution and Control of Emerging Viruses (2018RU009), Chinese Academy of Medical Sciences, Beijing, 102206, China. Electronic address: liujun@ivdc.chinacdc.cn.

PMID: 37384968
DOI: 10.1016/j.virol.2023.06.014

Abstract

Aichi virus C, a species in the genus Kobuvirus, causes diarrhea diseases in pigs and goats and pose health threat and economic loss for stock farming. A nearly complete genome sequence of caprine kobuvirus GCCDC14 was obtained from an anal swab of a black goat died from diarrhea collected in Hubei, China in 2019. Phylogenetic analyses suggested that GCCDC14 is a novel genotype of Aichi virus C, forming a sister branch to other caprine kobuviruses, with P1 and VP0 genes more closely related to porcine kobuviruses and VP3 in an independent branch. Compared to previous caprine kobuviruses, unique amino acid changes in the poly-l-proline type II helix structure of VP0 and VP1 were found, which may affect the cellular machinery of host and pathogenicity. This study indicates the presence of the kobuvirus with continuously evolving features and emphasizes the surveillance and genetic evolution investigation of kobuviruses for safety of husbandry.

Keywords: Caprine kobuvirus; China; Metagenomics sequencing; Molecular characteristics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Diarrhea
Feces
Genotype
Goats
Kobuvirus* / genetics
Phylogeny
Picornaviridae Infections* / epidemiology
Swine