Development of a synchronous recording and photo-stimulating electrode in multiple brain neurons

Qingping Zhang; Wei Jing; Shiping Wu; Mengzheng Zhu; Jingrui Jiang; Xiang Liu; Dian Yu; Long Cheng; Bin Feng; Jianbin Wen; Feng Xiong; Youming Lu; Huiyun Du

doi:10.3389/fnins.2023.1195095

Development of a synchronous recording and photo-stimulating electrode in multiple brain neurons

Front Neurosci. 2023 Jun 13:17:1195095. doi: 10.3389/fnins.2023.1195095. eCollection 2023.

Authors

Qingping Zhang^{1

2

3}, Wei Jing^{1

2

3}, Shiping Wu^{1

2

3}, Mengzheng Zhu^{1

2

3}, Jingrui Jiang^{1

2

3}, Xiang Liu^{1

2

3}, Dian Yu^{1

2

3}, Long Cheng^{1

2

3}, Bin Feng^{2

4}, Jianbin Wen^{1

2

3}, Feng Xiong⁵, Youming Lu^{1

2

3}, Huiyun Du^{1

2

6}

Affiliations

¹ Department of Physiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
² Wuhan Center for Brain Science, Huazhong University of Science and Technology, Wuhan, China.
³ Department of Pathophysiology, School of Basic Medicine, Huazhong University of Science and Technology, Wuhan, China.
⁴ Department of Neurobiology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
⁵ Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, National Center for Magnetic Resonance in Wuhan, Innovation Academy of Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, China.
⁶ Hubei Key Laboratory of Drug Target Research and Pharmacodynamic Evaluation, Wuhan, China.

Abstract

The investigation of brain networks and neural circuits involves the crucial aspects of observing and modulating neurophysiological activity. Recently, opto-electrodes have emerged as an efficient tool for electrophysiological recording and optogenetic stimulation, which has greatly facilitated the analysis of neural coding. However, implantation and electrode weight control have posed significant challenges in achieving long-term and multi-regional brain recording and stimulation. To address this issue, we have developed a mold and custom-printed circuit board-based opto-electrode. We report successful opto-electrode placement and high-quality electrophysiological recordings from the default mode network (DMN) of the mouse brain. This novel opto-electrode facilitates synchronous recording and stimulation in multiple brain regions and holds promise for advancing future research on neural circuits and networks.

Keywords: brain network; default mode network; electrophysiological recording; multichannel recording; neural circuit; optogenetic.