In vitro generation of a functional retinal pigment epithelium (RPE) monolayer sheet is useful and promising for RPE cell therapy. Here, we outline a method to construct engineered RPE sheets treated by induced pluripotent stem cell-conditioned medium (iPS-CM) in conjunction with femtosecond laser intrastromal lenticule (FLI-lenticule) scaffold to aid in enhanced RPE characteristics and cilium assembly. Such a strategy to construct RPE sheets is a promising avenue for developing RPE cell therapy, disease models, and drug screening tools.
Keywords: Corneal lenticule; Retinal pigment epithelial cells; Tissue engineering; iPS-CM.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.