Detoxification of p-nitrophenol (PNP) using Enterococcus gallinarum JT-02 isolated from animal farm waste sludge

Julieta Celeste Martín Tempestti; Harshavardhan Mohan; Pavithra Muthukumar Sathya; Se-Won Lee; Janaki Venkatachalam; Byung-Taek Oh; Kamala-Kannan Seralathan

doi:10.1016/j.envres.2023.116289

Detoxification of p-nitrophenol (PNP) using Enterococcus gallinarum JT-02 isolated from animal farm waste sludge

Environ Res. 2023 Aug 15;231(Pt 3):116289. doi: 10.1016/j.envres.2023.116289. Epub 2023 May 30.

Authors

Julieta Celeste Martín Tempestti¹, Harshavardhan Mohan², Pavithra Muthukumar Sathya³, Se-Won Lee³, Janaki Venkatachalam⁴, Byung-Taek Oh³, Kamala-Kannan Seralathan⁵

Affiliations

¹ Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Luján de Cuyo, Mendoza M5528AHB, Argentina; Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan, 54596, Jeonbuk, South Korea.
² Department of Chemistry, Research Institute of Physics and Chemistry, Jeonbuk National University, Jeonju, 54896, Republic of Korea.
³ Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan, 54596, Jeonbuk, South Korea.
⁴ PG and Research Department of Chemistry, Sri Sarada College for Women, Salem, 636016, Tamil Nadu, India.
⁵ Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University, Iksan, 54596, Jeonbuk, South Korea. Electronic address: kannan@jbnu.ac.kr.

PMID: 37263467
DOI: 10.1016/j.envres.2023.116289

Abstract

Enterococcus gallinarum (JT-02) isolated and identified from the animal farm waste sludge was found to be capable of biodegrading p-nitrophenol (PNP), an organic compound used to manufacture drugs, fungicides, insecticides, dyes, and to darken leather. The intention of this study was to optimize the biodegradation by finding the optimal conditions for the specific strain through single-factor experiments. The bacterial strain was grown in Luria Bertani broth and various parameters were optimized to achieve the prime settings for the p-nitrophenol (PNP) biodegradation. The results indicated that the best setups for the biodegradation by the strain JT-02 was 100 mg/L of PNP; pH 7; 30 °C; 150 rpm in a shaker incubator and 3% (v/v) of inoculum dose. Once the optimal conditions were found, the bacteria were capable of degrading p-nitrophenol (98.21%) in 4 days. Intermediates produced during PNP biodegradation were identified using High Performance Liquid Chromatography (HPLC) analysis and the biodegradation pathway was elucidated. Phytotoxicity studies were carried out with Vigna radiata seeds to confirm the applicability and efficiency of PNP biodegradation.

Keywords: Biodegradation; Enterococcus gallinarum; Phytotoxicity; p-nitrophenol.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteria / metabolism
Enterococcus*
Farms
Sewage* / microbiology

Substances

Sewage
4-nitrophenol

Supplementary concepts

Enterococcus gallinarum