TFAP2A promotes cervical cancer via a positive feedback pathway with PD‑L1

Oncol Rep. 2023 Jun;49(6):114. doi: 10.3892/or.2023.8551. Epub 2023 Apr 21.

Abstract

Transcription factor AP‑2 alpha (TFAP2A) is a critical cell growth regulator that is overexpressed in various tumor tissues. However, its role in the development of cervical cancer remains unknown. In the present study, public databases were thus explored and a higher expression of TFAP2A was found in cervical cancer. A total of 131 clinical samples were collected and it was also identified that TFAP2A was highly expressed in cervical tumor tissues. TFAP2A was also found to be associated with a higher tumor stage, lymph node metastasis and a poor patient survival. In vitro experiments revealed that the knockdown of TFAP2A inhibited the proliferation and migration of cervical cancer cells and promoted apoptosis. Furthermore, it was observed that TFAP2A could bind the programmed death‑ligand 1 (PD‑L1) promoter region and PD‑L1 rescued TFAP2A expression. In vivo experiments also revealed that TFAP2A promoted tumor growth. Collectively, in the present study it was demonstrated that TFAP2A is a transcription factor of PD‑L1 and a prognostic factor with clinical value, identifying a positive feedback loop of TFAP2A/PD‑L1.

Keywords: cervical cancer; migration; programmed death‑ligand 1; proliferation; transcription factor AP‑2 alpha.

MeSH terms

  • B7-H1 Antigen / genetics
  • Cell Line, Tumor
  • Cell Proliferation
  • Feedback
  • Female
  • Humans
  • Transcription Factor AP-2* / genetics
  • Transcription Factor AP-2* / metabolism
  • Uterine Cervical Neoplasms* / genetics

Substances

  • B7-H1 Antigen
  • CD274 protein, human
  • TFAP2A protein, human
  • Transcription Factor AP-2

Grants and funding

The present study was supported by the National Natural Science Foundation of China (grant no. 8197103302).