Structural characterization and immunomodulatory activity of a polysaccharide from finger citron extracted by continuous phase-transition extraction

Shuhui Wang; Yujie Yang; Qun Wang; Zhiqin Wu; Xiaojuan Liu; Shuxi Chen; Aimei Zhou

doi:10.1016/j.ijbiomac.2023.124491

Structural characterization and immunomodulatory activity of a polysaccharide from finger citron extracted by continuous phase-transition extraction

Int J Biol Macromol. 2023 Jun 15:240:124491. doi: 10.1016/j.ijbiomac.2023.124491. Epub 2023 Apr 17.

Authors

Shuhui Wang¹, Yujie Yang¹, Qun Wang¹, Zhiqin Wu¹, Xiaojuan Liu¹, Shuxi Chen², Aimei Zhou³

Affiliations

¹ Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
² Guangdong Zhancui Food Co., Ltd., Chaozhou 515634, China.
³ Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address: zhouam@scau.edu.cn.

PMID: 37076066
DOI: 10.1016/j.ijbiomac.2023.124491

Abstract

FCP-2-1, a water-soluble polysaccharide rich in galacturonic acid was isolated by continuous phase-transition extraction and purified with DEAE-52 cellulose and Sephadex G-100 column chromatography from finger citron with essential oil and flavonoids removed. The structural characterization and immunomodulatory activity of FCP-2-1 were further investigated in this work. FCP-2-1 with a M_w and M_n of 1.503 × 10⁴ g/mol and 1.125 × 10⁴ g/mol, respectively, was predominantly composed of galacturonic acid, galactose, and arabinose in a molar ratio of 0.685: 0.032: 0.283. The main linkage types of FCP-2-1 were proved to be →5)-α-L-Araf-(1→ and →4)-α-D-GalpA-(1→ based on methylation and NMR analysis. Moreover, FCP-2-1 was demonstrated to have significant immunomodulatory effects on macrophages in vitro by improving the cell viability, and enhancing phagocytic activity and secretion of NO and cytokines (IL-1β, IL-6, IL-10 and TNF-α), indicating that FCP-2-1 could be used as a natural agent in immunoregulation functional foods.

Keywords: Continuous phase-transition extraction; Finger citron polysaccharides; Immunomodulatory activity.

MeSH terms

Cytokines*
Hexuronic Acids / chemistry
Macrophages
Polysaccharides* / chemistry

Substances

galacturonic acid
Polysaccharides
Hexuronic Acids
Cytokines