Relating CYP2B6 genotype and efavirenz resistance among post-partum women living with HIV with high viremia in Uganda: a nested cross-sectional study

AIDS Res Ther. 2023 Mar 31;20(1):20. doi: 10.1186/s12981-023-00514-2.

Abstract

Background: We investigated the association between CYP2B6 polymorphisms and efavirenz drug resistance among women living with HIV who started on antiretroviral therapy during pregnancy and with high viremia during post-partum.

Methods: This was a cross-sectional study of women with viral loads greater than 1000 copies/ml who were at least 6 weeks postpartum. Sanger sequencing was used to detect resistant mutations, as well as host genotyping, and efavirenz resistance was compared among the metabolizer genotypes.

Results: Over the course of one year (July 2017-July 2018), 322 women were screened, with 110 (34.2%) having viral loads of 1000 copies/ml and 62 having whole blood available for genotyping. Fifty-nine of these women had both viral resistance and human host genotypic results. Efavirenz resistance according to metabolizer genotype was; 47% in slow, 34% in extensive and 28% in intermediate metabolizers, but the difference was not statistically significant due to the small sample size.

Conclusions: There was no statistically significant difference in EFV resistance between EFV metabolizer genotypes in women who started antiretroviral therapy during pregnancy and had high viremia in the postpartum period. However, a numerical trend was discovered, which calls for confirmation in a large, well-designed, statistically powered study.

Keywords: CYP2B6; HIV; HIV drug resistance; Post-partum; Pregnancy; Single nucleotide polymorphisms.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Anti-HIV Agents* / therapeutic use
  • Benzoxazines / therapeutic use
  • Cross-Sectional Studies
  • Cytochrome P-450 CYP2B6 / genetics
  • Female
  • Genotype
  • HIV Infections*
  • Humans
  • Polymorphism, Single Nucleotide
  • Postpartum Period
  • Pregnancy
  • Uganda / epidemiology
  • Viremia / drug therapy

Substances

  • Cytochrome P-450 CYP2B6
  • Anti-HIV Agents
  • efavirenz
  • Benzoxazines
  • CYP2B6 protein, human