An ultrasensitive label-free fluorescent aptamer sensor based on pH-gated release coumarin for detect HER2

Ning Li; Ya Zhang; Ying Xu; Xiaofang Liu; Wenhao Ma; Tingxiu Xiang; Changjun Hou; Danqun Huo

doi:10.1016/j.saa.2023.122641

An ultrasensitive label-free fluorescent aptamer sensor based on pH-gated release coumarin for detect HER2

Spectrochim Acta A Mol Biomol Spectrosc. 2023 Aug 5:296:122641. doi: 10.1016/j.saa.2023.122641. Epub 2023 Mar 17.

Authors

Ning Li¹, Ya Zhang¹, Ying Xu¹, Xiaofang Liu¹, Wenhao Ma¹, Tingxiu Xiang², Changjun Hou³, Danqun Huo⁴

Affiliations

¹ Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China.
² Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China. Electronic address: xiangtx1@cqu.edu.cn.
³ Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China; National Facility for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, PR China. Electronic address: houcj@cqu.edu.cn.
⁴ Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China; Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing 400044, PR China. Electronic address: huodq@cqu.edu.cn.

PMID: 36989691
DOI: 10.1016/j.saa.2023.122641

Abstract

Evaluation of human epidermal growth factor receptor 2 (HER2) molecular markers is a very suitable option for early diagnosis of breast cancer. Metal-organic frameworks (MOFs) have large porosity and surface interactions such as π-π stacking, electrostatics, hydrogen bonding, and coordination. Here, we integrated the HER2 aptamer and fluorescent probe coumarin (COU) with zeolite imidazolic acid framework-8 (ZIF-8) to construct a label-free fluorescent aptamer sensor with pH-gated release of COU. In the presence of the target-HER2, the aptamer adsorbed on the surface of ZIF-8@COU specifically recognizes and falls off the HER2 protein, exposing a portion of the pore size of ZIF-8@COU while reducing the negative charge on the sensor surface, under alkaline hydrolysis conditions, a large number of COU fluorescent molecules can be produced and released in the detection system.The aptamer fluorescence sensor has good detection performance, sensitivity and low background interference, the detection linearity range of HER2 protein is 0.05-10 ng/mL, the detection limit is 0.0005 ng/mL, and it has good recovery rate for the serum detection of clinical breast cancer patients. Therefore, this sensor has high potential in detecting and monitoring HER2 levels for the care and clinical diagnosis of breast cancer patients.

Keywords: Coumarin; HER2; Label-free aptamer sensor; Low background signal; Ph-gated release; ZIF-8.

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques*
Breast Neoplasms* / diagnosis
Coumarins
Female
Fluorescent Dyes
Humans
Hydrogen-Ion Concentration
Limit of Detection

Substances

Aptamers, Nucleotide
Fluorescent Dyes
Coumarins