Transcriptomics explores the potential of flavonoid in non-medicinal parts of Saposhnikovia divaricata (Turcz.) Schischk

Yuqiu Chen; Tao Zhang; Changbao Chen; Zhefeng Xu; Chunshuo Liu

doi:10.3389/fpls.2023.1067920

Transcriptomics explores the potential of flavonoid in non-medicinal parts of Saposhnikovia divaricata (Turcz.) Schischk

Front Plant Sci. 2023 Feb 27:14:1067920. doi: 10.3389/fpls.2023.1067920. eCollection 2023.

Authors

Yuqiu Chen¹, Tao Zhang¹, Changbao Chen¹, Zhefeng Xu¹, Chunshuo Liu¹

Affiliation

¹ Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, Jilin, China.

Abstract

Introduction: Saposhnikovia divaricata is a traditional Chinese medicine in China, which is widely used in clinic. The root of S. divaricata is often used as medicine, but little research has been done on its other tissues.

Methods: In this study, the contents of root and leaf of S. divaricata were determined by HPLC, the differentially expressed genes were screened by transcriptome sequencing at molecular level, and then verified by network pharmacology.

Results: The results showed that the content of 4'-O-β-D-glucosyl-5-O-methylvisamminol in the leaves was significantly higher than that in the roots, which was about 3 times higher than that in the roots. In addition, 10 differentially expressed key enzyme genes were screened in plant hormone signal transduction, phenylpropanoid and flavonoid biosynthetic pathways. C4H and CYP98A were up-regulated in root, while F3H was down-regulated in root. They can be used as important candidate genes for the mechanism of quality difference of S. divaricata. Finally, network pharmacological validation showed that 5-O-methylvesamitol plays an important role in the treatment of ulcerative colitis.

Discussion: These findings not only provide insight into flavonoid biosynthesis in S. divaricata associated molecular regulation, but also provide a theoretical basis for the development and utilization of S. divaricata.

Keywords: 5-O-methylvesamitol; Saposhnikovia divaricata; flavonoid biosynthesis; network pharmacology; transcriptome.

Grants and funding

This work was supported by the National Key R&D projects of China (2019YFC1710704) and Key projects of the science and technology development plan of Jilin province, China (20200708028YY).