Rapid diagnostics for skin and soft tissue infections: the current landscape and future potential

Curr Opin Infect Dis. 2023 Apr 1;36(2):57-66. doi: 10.1097/QCO.0000000000000901. Epub 2023 Jan 24.

Abstract

Purpose of review: Managing antimicrobial therapy in patients with complicated skin and soft tissue infections (SSTI) constitutes a growing challenge due to the wide spectrum of potential pathogens and resistance phenotypes. Today, microbiological documentation relies on cultural methods. This review summarizes the available evidence regarding the clinical input of rapid microbiological diagnostic tools (RMDT) and their impact on the management of antimicrobial therapy in SSTI.

Recent findings: Accurate tools are already available for the early detection of methicillin-resistant Staphylococcus aureus (MRSA) in SSTI samples and may help avoiding or shortening empirical anti-MRSA coverage. Further research is necessary to develop and evaluate RMDT detecting group A streptococci (e.g., antigenic test) and Gram-negative pathogens (e.g., multiplex PCR assays), including through point-of-care utilization. Next-generation sequencing (NGS) methods could provide pivotal information for the stewardship of antimicrobial therapy, especially in case of polymicrobial or fungal SSTI and in the immunocompromised host; however, a shortening in the turnaround time and prospective data regarding their therapeutic input are needed to better appraise the clinical positioning of these promising approaches.

Summary: The clinical input of RMDT in SSTI is currently limited due to the scarcity of available dedicated assays and the polymicrobial feature of certain cases. NGS appears as a relevant tool but requires further developments before its implementation in routine clinical practice.

Publication types

  • Review

MeSH terms

  • Anti-Bacterial Agents / therapeutic use
  • Humans
  • Methicillin-Resistant Staphylococcus aureus* / genetics
  • Prospective Studies
  • Rapid Diagnostic Tests
  • Skin
  • Soft Tissue Infections* / diagnosis
  • Soft Tissue Infections* / drug therapy
  • Soft Tissue Infections* / microbiology
  • Staphylococcal Infections* / drug therapy
  • Staphylococcal Skin Infections* / drug therapy

Substances

  • Anti-Bacterial Agents