Effect of BaltPLA2, a phospholipase A2 from Bothrops alternatus snake venom, on the viability of cells infected with dengue virus

Edigar Henrique Vaz Dias; Bruna Barbosa de Sousa Simamoto; Déborah Fernanda da Cunha Pereira; Mariana Santos Matias Ribeiro; Fernanda Maria Santiago; Fábio de Oliveira; Jonny Yokosawa; Carla Cristine Neves Mamede

doi:10.1016/j.tiv.2023.105562

Effect of BaltPLA₂, a phospholipase A₂ from Bothrops alternatus snake venom, on the viability of cells infected with dengue virus

Toxicol In Vitro. 2023 Apr:88:105562. doi: 10.1016/j.tiv.2023.105562. Epub 2023 Jan 20.

Authors

Edigar Henrique Vaz Dias¹, Bruna Barbosa de Sousa Simamoto², Déborah Fernanda da Cunha Pereira³, Mariana Santos Matias Ribeiro³, Fernanda Maria Santiago⁴, Fábio de Oliveira⁵, Jonny Yokosawa², Carla Cristine Neves Mamede⁶

Affiliations

¹ Biotechnology Institute, Federal University of Catalão (UFCAT), Catalão, Goiás, Brazil. Electronic address: edigar.henrique@ufcat.edu.br.
² Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil.
³ Institute of Genetics and Biochemistry, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil.
⁴ Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil. Electronic address: fmsantiago@ufu.br.
⁵ Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil. Electronic address: fabio@ufu.br.
⁶ Institute of Biomedical Sciences, Federal University of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil. Electronic address: carlamamede@ufu.br.

PMID: 36690282
DOI: 10.1016/j.tiv.2023.105562

Abstract

Dengue fever is considered a major public health problem in tropical and subtropical regions. Our study analyzed the effect of BaltPLA_2, a phospholipase A₂ from Bothrops alternatus snake venom, on the viability of cells infected with Dengue virus. In presence of BaltPLA₂, the viability of infected cells increased significantly in virucidal, post-treatment, and adsorption assays. Although preliminary these results reveal the need for further studies to investigated whether BaltPLA₂ has antiviral activity against Dengue virus.

Keywords: Antiviral; Dengue virus; Snake toxins.

MeSH terms

Animals
Antiviral Agents / pharmacology
Bothrops*
Crotalid Venoms* / pharmacology
Dengue Virus*
Phospholipases A2 / pharmacology
Snake Venoms

Substances

Phospholipases A2
Snake Venoms
Antiviral Agents
Crotalid Venoms