Ferroptosis is involved in PM2.5-induced acute nasal epithelial injury via AMPK-mediated autophagy

Abstract

PM2.5 is one of the main harmful environmental pollutants and can damage nasal epithelial carriers to worsen allergic rhinitis. Ferroptosis is a novel form of regulated cell death with iron-dependent lipid peroxidation. However, whether ferroptosis is involved in PM2.5-induced nasal epithelial injury has not been elucidated. To verify the vital role of ferroptosis in PM2.5-induced nasal epithelial injury and further explore the potential mechanism, we detected intracellular iron content, ROS release and lipid peroxidation and ferroptosis-related proteins in vitro as well as the pathological changes in the nasal epithelium and the levels of proinflammatory factors in nasal lavage fluid in vivo. Our results showed that PM2.5 exposure led to oxidative stress, labile iron accumulation and lipid peroxidation in HNEPCs. In addition, the expression levels of xCT, GPx4, FTH1 and FTL in HNEPCs were greatly inhibited by PM2.5. Treatment with the ferroptosis inhibitors deferoxamine (DFO) and ferrostatin-1 (Fer-1) significantly reversed the toxicity of PM2.5 to human nasal epithelial cells (HNEPCs). Mechanistically, AMPK-mediated autophagy was initiated during PM2.5 exposure, which drove ferroptosis of HNEPCs. Autophagy inhibitor remarkably improved cell death, oxidative stress, labile iron accumulation, lipid peroxidation, and the downregulated expression of xCT, GPx4, FTH1 and FTL in HNEPCs induced by PM2.5. Furthermore, an AMPK inhibitor (Compound C, CC) and siRNA-AMPKα suppressed autophagy activation and ferroptosis stimulated by PM2.5. In vivo, Fer-1 reduced nasal epithelial injury and mucus secretion in PM2.5-exposed mice. In addition, CC significantly improved nasal epithelial damage and proinflammatory factor production in mice caused by PM2.5 intranasal treatment. In addition, CC greatly inhibited autophagy activation but reversed the downregulation of GPX4 and FTH1 induced by PM2.5 in the nasal epithelium of mice. Together, these data suggest that AMPK-mediated autophagy plays an important role in PM2.5-induced ferroptosis and that AMPK might be a potential treatment target for PM2.5-induced nasal epithelial injury.

Keywords: AMPK; Autophagy; Ferroptosis; Nasal epithelium; PM2.5.