FOXG1 Contributes Adult Hippocampal Neurogenesis in Mice

Int J Mol Sci. 2022 Nov 29;23(23):14979. doi: 10.3390/ijms232314979.

Abstract

Strategies to enhance hippocampal precursor cells efficiently differentiate into neurons could be crucial for structural repair after neurodegenerative damage. FOXG1 has been shown to play an important role in pattern formation, cell proliferation, and cell specification during embryonic and early postnatal neurogenesis. Thus far, the role of FOXG1 in adult hippocampal neurogenesis is largely unknown. Utilizing CAG-loxp-stop-loxp-Foxg1-IRES-EGFP (Foxg1fl/fl), a specific mouse line combined with CreAAV infusion, we successfully forced FOXG1 overexpressed in the hippocampal dentate gyrus (DG) of the genotype mice. Thereafter, we explored the function of FOXG1 on neuronal lineage progression and hippocampal neurogenesis in adult mice. By inhibiting p21cip1 expression, FOXG1-regulated activities enable the expansion of the precursor cell population. Besides, FOXG1 induced quiescent radial-glia like type I neural progenitor, giving rise to intermediate progenitor cells, neuroblasts in the hippocampal DG. Through increasing the length of G1 phase, FOXG1 promoted lineage-committed cells to exit the cell cycle and differentiate into mature neurons. The present results suggest that FOXG1 likely promotes neuronal lineage progression and thereby contributes to adult hippocampal neurogenesis. Elevating FOXG1 levels either pharmacologically or through other means could present a therapeutic strategy for disease related with neuronal loss.

Keywords: Alzheimer’s disease; FOXG1; cell cycle; hippocampus; neural stem cell.

MeSH terms

  • Animals
  • Cell Proliferation
  • Forkhead Transcription Factors / genetics
  • Forkhead Transcription Factors / metabolism
  • Hippocampus / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Neural Stem Cells* / metabolism
  • Neurogenesis* / genetics
  • Neurons / metabolism

Substances

  • Foxg1 protein, mouse
  • Nerve Tissue Proteins
  • Forkhead Transcription Factors