Evaluation of microaneurysms as predictors of therapeutic response to anti-VEGF therapy in patients with DME

PLoS One. 2022 Nov 28;17(11):e0277920. doi: 10.1371/journal.pone.0277920. eCollection 2022.

Abstract

Administration of intravitreal anti-vascular endothelial growth factor (anti-VEGF) therapy is the first-line therapy for diabetic macular oedema (DME). However, some patients show no or insufficient response to repeated anti-VEGF injections. Therefore, it is necessary to identify factors that can predict this resistance against anti-VEGF treatment. Presence of microaneurysms (MAs) is a predictor of the development and progression of DME, but its relationship with the treatment response to the anti-VEGF agents is not well known. Therefore, we aimed to elucidate the relationship between the distribution of MAs and the response to anti-VEGF therapy in patients with DME. The number of MAs was measured before anti-VEGF therapy in each region using fluorescein angiography, indocyanine green angiography (IA), and optical coherence tomography angiography. Patients with DME were divided into the responder and non-responder groups after three loading phases. Differences in the distribution of MAs between the groups were investigated. Pre-treatment IA revealed more MAs in the nasal area in the non-responder group than in the responder group (10.7 ± 10.7 and 5.7 ± 5.7, respectively, in the nasal macula) (1.4 ± 2.1 and 0.4 ± 0.7, respectively, in the nasal fovea). Whereas, pre-treatment FA and OCTA could not reveal significantly difference between the groups. Detection of MAs in the nasal macula using pre-treatment IA may indicate resistance to anti-VEGF therapy. We recommend the clinicians confirm the presence of MAs in the nasal macula, as shown by IA, as a predictor of therapeutic response to anti-VEGF therapy in patients with treatment naive DME.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescein Angiography
  • Humans
  • Macula Lutea*
  • Macular Edema* / diagnostic imaging
  • Macular Edema* / drug therapy
  • Microaneurysm* / diagnostic imaging
  • Microaneurysm* / drug therapy
  • Vascular Endothelial Growth Factors

Substances

  • Vascular Endothelial Growth Factors

Grants and funding

Kazuhiro Kimura, the principal investigator, signed a contract to receive 21,795,000 yen from Novartis Pharma for using the milestone method. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of manuscript.