Mixotrophic culture of bait microalgae for biomass and nutrients accumulation and their synergistic carbon metabolism

Yahui Bo; Ruirui Chu; Danni Sun; Xiangyuan Deng; Chengxu Zhou; Xiaojun Yan; Roger Ruan; Pengfei Cheng

doi:10.1016/j.biortech.2022.128301

Mixotrophic culture of bait microalgae for biomass and nutrients accumulation and their synergistic carbon metabolism

Bioresour Technol. 2023 Jan:367:128301. doi: 10.1016/j.biortech.2022.128301. Epub 2022 Nov 9.

Authors

Yahui Bo¹, Ruirui Chu¹, Danni Sun¹, Xiangyuan Deng², Chengxu Zhou¹, Xiaojun Yan³, Roger Ruan⁴, Pengfei Cheng⁵

Affiliations

¹ College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang, 315211, China.
² College of Biotechnology, Jiangsu University of Science and Technology, 212100 Zhenjiang, China.
³ Key Laboratory of Marine Biotechnology of Zhejiang Province, Ningbo University, Ningbo, Zhejiang 315211, China.
⁴ Center for Biorefining, and Department of Bioproducts and Biosystems Engineering, University of Minnesota-Twin Cities, Saint Paul, MN 55108, USA.
⁵ College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo, Zhejiang, 315211, China. Electronic address: chengpengfei@nbu.edu.cn.

PMID: 36370937
DOI: 10.1016/j.biortech.2022.128301

Abstract

Microalgae cannot meet the bait demand for aquaculture due to light intensity limitation and other disadvantageous conditions. This research selected 6 mixotrophic microalgae, and the optimal strains and organic carbon were screened. The results showed that Thalassiosira pseudonana and Chlorella sp. are suitable for mixotrophic culture. The maximum cell density of Thalassiosira pseudonana was found to be 1.67 times than that of the photoautotrophic group when glycerol was added. The maximum cell density of Chlorella sp. with acetic acid was 1.69 times than that of the photoautotrophic group. When the concentration of acetic acid was 5.0 g·L^-1 and the concentration of KNO₃ was 0.2 g·L^-1, the maximum biomass of Chlorella sp. could reach 3.54 × 10⁷ cells·mL^-1; the maximum biomass of Thalassiosira pseudonana was 5.53 × 10⁶ cells·mL^-1 with 10.0 g·L^-1 glycerol and 0.2 g·L^-1 KNO₃. Metabolomic analysis further revealed that mixotrophic bait microalgae could promote the accumulation of lipids and amino acids.

Keywords: Bait microalgae; Culture conditions; Metabolomic analysis; Mixotrophic culture; Organic carbon.

MeSH terms

Acetates / metabolism
Biomass
Carbon / metabolism
Chlorella* / metabolism
Glycerol / metabolism
Microalgae* / metabolism
Nutrients

Substances

Carbon
Glycerol
Acetates