Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)

Hui Lin; Shi-Qiao Ye; Zhao-Hui Xu; Jacqueline S Penaloza; Mona Aljuhani; Tatyana Vetter; Ming-Tao Zhao; Kim L McBride

doi:10.1016/j.scr.2022.102958

Generation and characterization of a human induced pluripotent stem cell (iPSC) line from a patient with congenital heart disease (CHD)

Stem Cell Res. 2022 Dec:65:102958. doi: 10.1016/j.scr.2022.102958. Epub 2022 Oct 31.

Authors

Hui Lin¹, Shi-Qiao Ye¹, Zhao-Hui Xu², Jacqueline S Penaloza³, Mona Aljuhani¹, Tatyana Vetter⁴, Ming-Tao Zhao⁵, Kim L McBride⁶

Affiliations

¹ Center for Cardiovascular Research and The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA.
² Center for Vaccines and Immunity, Nationwide Children's Hospital, Columbus, OH, USA; Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA.
³ Computational Genomics Group, The Steve and Cindy Rasmussen Institute for Genomic Medicine, Nationwide Children's Hospital, Columbus, OH, USA.
⁴ Center for Gene Therapy, Nationwide Children's Hospital, Columbus, OH, USA.
⁵ Center for Cardiovascular Research and The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA; Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA.
⁶ Center for Cardiovascular Research and The Heart Center, Nationwide Children's Hospital, Columbus, OH, USA; Department of Pediatrics, College of Medicine, The Ohio State University, Columbus, OH, USA. Electronic address: Kim.mcbride@nationwidechildrens.org.

Abstract

Epstein-Barr virus (EBV) immortalized lymphoblastoid cell lines (LCLs) are widely used for banking. This bioresource could be leveraged for creating human iPSC lines to model diseases including CHD. We generated an LCL-derived iPSC line (NCHi001-A) from a patient with congenital aortic valve stenosis. NCHi001-A was EBV and transgenes free, exhibited stem cell-like morphology, expressed pluripotency markers, has a normal karyotype, and could be differentiated into cells of three germ layers in vitro. Relationship inference via a microarray-based analysis showed NCHi001-A is identical to the parental cell line. NCHi001-A can be used for disease modeling, drug discovery, and cell therapy development.

Publication types

Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

MeSH terms

Epstein-Barr Virus Infections*
Heart Defects, Congenital* / genetics
Herpesvirus 4, Human
Humans
Induced Pluripotent Stem Cells*

Abstract

Publication types

MeSH terms

Grants and funding