A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry

Zhengyuan Pang; Li Ye

doi:10.1016/j.xpro.2022.101778

A protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution using tissue clearing and click chemistry

STAR Protoc. 2022 Oct 25;3(4):101778. doi: 10.1016/j.xpro.2022.101778. eCollection 2022 Dec 16.

Authors

Zhengyuan Pang¹, Li Ye²

Affiliations

¹ Department of Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA. Electronic address: zypang@scripps.edu.
² Department of Neuroscience, The Scripps Research Institute, La Jolla, CA 92037, USA; Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA. Electronic address: liye@scripps.edu.

Abstract

Here, we provide a protocol to visualize on-target specific drug binding in mammalian tissue with cellular resolution. By combining tissue clearing and click chemistry, this protocol allows fluorescence tagging of covalent drug binding in situ. In addition, the protocol is compatible with molecular marker staining for cell type identifications. For complete details on the use and execution of this protocol, please refer to Pang et al. (2022).

Keywords: Biotechnology and bioengineering; Neuroscience.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Click Chemistry* / methods
Mammals*
Staining and Labeling

Abstract

Publication types

MeSH terms

Grants and funding