Single-Cell Transcriptome Analysis of Treg

Benjy Jek Yang Tan; Masahiro Ono; Yorifumi Satou

doi:10.1007/978-1-0716-2647-4_17

Single-Cell Transcriptome Analysis of Treg

Methods Mol Biol. 2023:2559:259-278. doi: 10.1007/978-1-0716-2647-4_17.

Authors

Benjy Jek Yang Tan¹, Masahiro Ono^{2

3}, Yorifumi Satou⁴

Affiliations

¹ Division of Genomics & Transcriptomics, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Japan.
² Department of Life Sciences, Imperial College London, London, UK.
³ IRCMS, Kumamoto University, Kumamoto, Japan.
⁴ Division of Genomics & Transcriptomics, Joint Research Center for Human Retrovirus Infection, Kumamoto University, Kumamoto, Japan. y-satou@kumamoto-u.ac.jp.

PMID: 36180638
DOI: 10.1007/978-1-0716-2647-4_17

Abstract

Regulatory T-cells (Treg) are considerably heterogeneous. Thymically derived Treg (tTreg) are those, which differentiate in the thymus, while peripherally derived Treg (pTreg) differentiate from peripheral mature CD4⁺ T-cells. These two populations are often identified using markers such as neuropilin-1 and Helios (for tTreg) and ROR-γt (for pTreg) in intestines (Tanoue et al., Nat Rev Immunol 16:295-309, 2016).

Keywords: Single-cell RNA-seq (scRNA-seq); Transcriptome; Treg.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Forkhead Transcription Factors / genetics
Gene Expression Profiling
Neuropilin-1 / genetics
Nuclear Receptor Subfamily 1, Group F, Member 3*
T-Lymphocytes, Regulatory*

Substances

Forkhead Transcription Factors
Nuclear Receptor Subfamily 1, Group F, Member 3
Neuropilin-1

Grants and funding

MR/S000208/1/MRC_/Medical Research Council/United Kingdom