In Vivo Detection of Cyclic-di-AMP in Staphylococcus aureus

Nagaraja Mukkayyan; Raymond Poon; Philipp N Sander; Li-Yin Lai; Zahra Zubair-Nizami; Ming C Hammond; Som S Chatterjee

doi:10.1021/acsomega.2c04538

In Vivo Detection of Cyclic-di-AMP in Staphylococcus aureus

ACS Omega. 2022 Aug 26;7(36):32749-32753. doi: 10.1021/acsomega.2c04538. eCollection 2022 Sep 13.

Authors

Nagaraja Mukkayyan^{1

2}, Raymond Poon^{1

2}, Philipp N Sander³, Li-Yin Lai^{1

2}, Zahra Zubair-Nizami^{1

2}, Ming C Hammond^{3

4}, Som S Chatterjee^{1

2

5}

Affiliations

¹ Department of Microbial Pathogenesis, School of Dentistry, University of Maryland, Baltimore, Maryland 21202, United States.
² Institute of Marine and Environmental Technology, Baltimore, Maryland 21202, United States.
³ Department of Chemistry, University of California, Berkeley, California 94720, United States.
⁴ Department of Chemistry and Henry Eyring Center for Cell and Genome Sciences, University of Utah, Salt Lake City, Utah 84112, United States.
⁵ University of Maryland Center for Environmental Science, Baltimore, Maryland 21202, United States.

Abstract

Cyclic-di-AMP (CDA) is a signaling molecule that controls various cellular functions including antibiotic tolerance and osmoregulation in Staphylococcus aureus (S. aureus). In this study, we developed a novel biosensor (bsuO P6-4) for in vivo detection of CDA in S. aureus. The fluorescent biosensor is based on a natural CDA riboswitch from Bacillus subtilis connected at its P6 stem to the dye-binding aptamer Spinach. Our study showed that bsuO P6-4 could detect a wide concentration range of CDA in both laboratory and clinical strains, making it suitable for use in both basic and clinical research applications.

Abstract

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