Phenotype-genotype analysis of Latilactobacills curvatus from different niches: Carbohydrate metabolism, antibiotic resistance, bacteriocin, phage fragments and linkages with CRISPR-Cas systems

Leilei Yu; Xiaojie Zang; Ying Chen; Yuhang Gao; Zhangming Pei; Bo Yang; Hao Zhang; Arjan Narbad; Fengwei Tian; Qixiao Zhai; Wei Chen

doi:10.1016/j.foodres.2022.111640

Phenotype-genotype analysis of Latilactobacills curvatus from different niches: Carbohydrate metabolism, antibiotic resistance, bacteriocin, phage fragments and linkages with CRISPR-Cas systems

Food Res Int. 2022 Oct:160:111640. doi: 10.1016/j.foodres.2022.111640. Epub 2022 Jul 9.

Authors

Leilei Yu¹, Xiaojie Zang², Ying Chen², Yuhang Gao², Zhangming Pei², Bo Yang¹, Hao Zhang³, Arjan Narbad⁴, Fengwei Tian⁵, Qixiao Zhai¹, Wei Chen³

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Research Laboratory for Probiotics at Jiangnan University, Wuxi, Jiangsu 214122, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Research Laboratory for Probiotics at Jiangnan University, Wuxi, Jiangsu 214122, China.
⁴ International Joint Research Laboratory for Probiotics at Jiangnan University, Wuxi, Jiangsu 214122, China; Gut Health and Microbiome Institute Strategic Programme, Quadram Institute Bioscience, Norwich 16 NR4 7UQ, UK.
⁵ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Research Laboratory for Probiotics at Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: fwtian@jiangnan.edu.cn.

PMID: 36076376
DOI: 10.1016/j.foodres.2022.111640

Abstract

The potential probiotic function of Latilactobacills curvatus has attracted the attention of researchers. To explore the differences in the genomes of L. curvatus, nine strains were isolated from various sources, including feces and fermented vegetables and compared with 25 strains from the NCBI database. The findings indicated that the average genome size, GC content, and CDS of L. curvatus were 1.94 MB, 41.9%, and 1825, respectively. Its core genome is associated with transcription, translation, carbohydrate transport and metabolism, and defense functions. The pan-genome of L. curvatus was in a closed state. The genetic diversity of L. curatus is mainly manifested in its ability to use carbohydrates, antibiotic resistance, bacteriocin operon, and polymeric regularly interspaced short palindromic repeats (CRISPR)-Cas for bacterial immunity. The CRISPR system of 34 strains of L. curvatus was predominantly found to be of the IIA type with a few IIC and IE types. These findings will contribute to a better understanding of this species.

Keywords: Antibiotic resistance; CRISPR-Cas; Carbohydrate utilization; Comparative genomics; Latilactobacills curvatus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteriocins* / genetics
Bacteriophages* / genetics
CRISPR-Cas Systems
Carbohydrate Metabolism / genetics
Drug Resistance, Microbial
Genome, Bacterial / genetics
Genotype
Phenotype

Substances

Bacteriocins

Abstract

Publication types

MeSH terms

Substances

Grants and funding