[Gene Sequencing Analyses of 10 ABO Ambiguous Blood Group Samples]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2022 Aug;30(4):1193-1197. doi: 10.19746/j.cnki.issn.1009-2137.2022.04.034.
[Article in Chinese]

Abstract
in English, Chinese

Objective: Through analysis of ABO blood group gene typing technology, to assist in the identification of difficult clinical serological specimens.

Methods: A total of 10 forwardreverse typing ambiguous samples were collected from January 2021 to August 2021 in our hospital.ABO genotypes were analysed by gene sequencing.

Results: The genotypes of 10 ABO ambiguous blood group samples were A102/BW11, A102/BW12, O02/O02, A102/B303, A102/B101, BW11/O02, B101/O04, BW11/O01, BW11/O01, A101/O02, respectively. The genotype results of 6 cases was consistent with the serological phenotype, and the serological phenotype of 4 cases were different from the geno sequencing.

Conclusion: ABO blood groups genotyping technology combined with serological typing can be used for accurate typing of ambiguous blood group, and better ensure the safety of blood transfusion.

题目: 10例ABO疑难血型的基因测序分析.

目的: 利用ABO血型基因分型技术协助临床血清学疑难血型的鉴定.

方法: 收集2021年1月－2021年8月本院用血清学鉴定ABO正反定型不符的疑难血型标本10例，采用基因测序方法检测ABO基因型.

结果: 10例疑难血型标本的ABO基因型分别为A102/Bw11、A102/Bw12、O02/O02、A102/B303、A102/B101、Bw11/O02、B101/O04、Bw11/O01、Bw11/O01、A101/O02。其中有6例标本血型基因测序结果和血清学表型一致，其他4例标本的血清学表型和基因测序结果均表现出一定的差异.

结论: 鉴定ABO疑难血型可利用基因分型技术结合血清学方法，最终准确鉴定血型，更好的保障输血安全.

Keywords: ABO blood group; ambiguous blood group; gene sequencing.

MeSH terms

  • ABO Blood-Group System* / genetics
  • Alleles
  • Blood Grouping and Crossmatching*
  • Exons
  • Genotype
  • Phenotype

Substances

  • ABO Blood-Group System