Generating patient-derived ascites-dependent xenograft mouse models of peritoneal carcinomatosis

Ying Liu; Wai Har Ng; Hong-Yuan Zhu; Qiu Xuan Tan; Josephine Hendrikson; Joey Wee-Shan Tan; Gillian Ng; Weng Khong Lim; Chin-Ann Johnny Ong

doi:10.1016/j.xpro.2022.101548

Generating patient-derived ascites-dependent xenograft mouse models of peritoneal carcinomatosis

STAR Protoc. 2022 Sep 16;3(3):101548. doi: 10.1016/j.xpro.2022.101548. Epub 2022 Jul 16.

Authors

Ying Liu¹, Wai Har Ng¹, Hong-Yuan Zhu¹, Qiu Xuan Tan¹, Josephine Hendrikson¹, Joey Wee-Shan Tan¹, Gillian Ng¹, Weng Khong Lim², Chin-Ann Johnny Ong³

Affiliations

¹ Department of Sarcoma, Peritoneal and Rare Tumors (SPRinT), Division of Surgery and Surgical Oncology, National Cancer Centre Singapore, Singapore 169610, Singapore; Department of Sarcoma, Peritoneal and Rare Tumors (SPRinT), Division of Surgery and Surgical Oncology, Singapore General Hospital, Singapore 169608, Singapore; Laboratory of Applied Human Genetics, Division of Medical Sciences, National Cancer Centre Singapore, Singapore 169610, Singapore.
² SingHealth Duke-NUS Institute of Precision Medicine, National Heart Centre Singapore, Singapore 169609, Singapore; Cancer and Stem Biology Signature Research Program, Duke-NUS Medical School, Singapore 169857, Singapore.
³ Department of Sarcoma, Peritoneal and Rare Tumors (SPRinT), Division of Surgery and Surgical Oncology, National Cancer Centre Singapore, Singapore 169610, Singapore; Department of Sarcoma, Peritoneal and Rare Tumors (SPRinT), Division of Surgery and Surgical Oncology, Singapore General Hospital, Singapore 169608, Singapore; Laboratory of Applied Human Genetics, Division of Medical Sciences, National Cancer Centre Singapore, Singapore 169610, Singapore; SingHealth Duke-NUS Oncology Academic Clinical Program, Duke-NUS Medical School, Singapore 169857, Singapore; SingHealth Duke-NUS Surgery Academic Clinical Program, Duke-NUS Medical School, Singapore 169857, Singapore; Institute of Molecular and Cell Biology, A∗STAR Research Entities, Singapore 138673, Singapore. Electronic address: johnny.ong.c.a@singhealth.com.sg.

Abstract

Clinically relevant animal models are crucial for effective development of therapeutics for peritoneal carcinomatosis (PC). This protocol describes the generation of patient-derived ascites-dependent xenograft (PDADX) models from the cellular component of ascites. The use of routine intraperitoneal injection of the fluid component of ascites is analogous to the biological events occurring intra-abdominally in patients with PC. By serving as a proxy, PDADX models represent a valuable tool for preclinical testing of new therapeutics for PC. For complete details on the use and execution of this protocol, please refer to Hendrikson et al. (2022).

Keywords: Cancer; Health Sciences; Model Organisms; Molecular Biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Ascites / drug therapy
Disease Models, Animal
Heterografts
Humans
Injections, Intraperitoneal
Mice
Peritoneal Neoplasms* / drug therapy