The Protein-Rich Fraction from Spirulina platensis Exerts Neuroprotection in Hemiparkinsonian Rats by Decreasing Brain Inflammatory-Related Enzymes and Glial Fibrillary Acidic Protein Expressions

J Med Food. 2022 Jul;25(7):695-709. doi: 10.1089/jmf.2021.0100.

Abstract

Spirulina platensis is a cyanobacterium with high protein content and presenting neuroprotective effects. Now, we studied a protein-enriched fraction (SPF), on behavior, neurochemical and immunohistochemical (IHC) assays in hemiparkinsonian rats, distributed into the groups: SO (sham-operated), 6-hydroxydopamine (6-OHDA), and 6-OHDA (treated with SPF, 5 and 10 mg/kg, p.o., 15 days). Afterward, animals were subjected to behavioral tests and euthanized, and brain areas used for neurochemical and IHC assays. SPF partly reversed the changes in the apomorphine-induced rotations, open field and forced swim tests, and also the decrease in striatal dopamine and 3,4-dihydroxyphenylacetic acid contents seen in hemiparkinsonian rats. Furthermore, SPF reduced brain oxidative stress and increased striatal expressions of tyrosine hydroxylase and dopamine transporter and significantly reduced hippocampal inducible nitric oxide synthase, cyclooxygenase-2 and glial fibrillary acidic protein expressions. The data suggest that the protein fraction from S. platensis, through its brain anti-inflammatory and antioxidative actions, exerts neuroprotective effects that could benefit patients affected by neurodegenerative diseases, like Parkinson's disease.

Keywords: Spirulina platensis; hemiparkinsonian rats; immunohistochemical assays; neuroprotection; oxidative stress.; phycocyanin.

MeSH terms

  • Animals
  • Brain / drug effects
  • Corpus Striatum / drug effects
  • Disease Models, Animal
  • Glial Fibrillary Acidic Protein / metabolism
  • Neuroprotection
  • Neuroprotective Agents* / metabolism
  • Neuroprotective Agents* / pharmacology
  • Neuroprotective Agents* / therapeutic use
  • Oxidopamine
  • Parkinson Disease* / drug therapy
  • Parkinson Disease* / metabolism
  • Rats
  • Spirulina* / metabolism
  • Tissue Extracts* / metabolism
  • Tissue Extracts* / pharmacology
  • Tissue Extracts* / therapeutic use

Substances

  • Glial Fibrillary Acidic Protein
  • Neuroprotective Agents
  • Tissue Extracts
  • Oxidopamine

Supplementary concepts

  • Arthrospira platensis