Investigating the Antimicrobial Activity of Vancomycin-Loaded Soy Protein Nanoparticles

Hadi Zare-Zardini; Hossein Soltaninejad; Adel Ghorani-Azam; Mohammad Javad Forouzani-Moghaddam; Sima Mozafri; Zohreh Akhoundi-Meybodi; Farzad Ferdosian; Fatemeh Jabinian

doi:10.1155/2022/5709999

Investigating the Antimicrobial Activity of Vancomycin-Loaded Soy Protein Nanoparticles

Interdiscip Perspect Infect Dis. 2022 Jun 29:2022:5709999. doi: 10.1155/2022/5709999. eCollection 2022.

Authors

Hadi Zare-Zardini^{1

2

3}, Hossein Soltaninejad⁴, Adel Ghorani-Azam⁵, Mohammad Javad Forouzani-Moghaddam⁶, Sima Mozafri², Zohreh Akhoundi-Meybodi², Farzad Ferdosian², Fatemeh Jabinian⁷

Affiliations

¹ Department of Biomedical Engineering, Meybod University, Meybod, Iran.
² Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
³ Medical Nanotechnology & Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
⁴ Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, Iran.
⁵ Department of Forensic Medicine and Toxicology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
⁶ School of Paramedical, Iran University of Medical Sciences, Tehran, Iran.
⁷ Department of Operating Room and Anesthesiology, School of Allied Medical Sciences, Tehran, Iran.

Abstract

Developing targeted and slow-release antibiotic delivery systems can effectively reduce drug overdose and side effects. This study aimed to investigate the antimicrobial activity of vancomycin-loaded soy protein nanoparticles (vancomycin-SPNs). For the preparation of SPNs, the desolvation method was applied in different concentrations of vancomycin and soy protein (15:5, 10:15, 6:20, 8:25, and 10:30 of vancomycin:soy protein). Scanning electron microscope (SEM), transmission electron microscopy (TEM), dynamic light scattering (DLS), and FTIR were used for nanoparticle characterization. Antibacterial activity was evaluated by the radial diffusion assay (RDA) and absorbance methods. Proper synthesis was demonstrated by characterization. The best drug loading (% entrapment efficiency = 90.2%), the fastest release rate (% release = 88.2%), and the best antibacterial activity were observed in ratio 10:30 of vancomycin:SPNs. Results showed that SPNs are a potent delivery system for antibiotic loading and slow release to control antibiotic use.