Serum and breastmilk SARS-CoV-2 specific antibodies following BNT162b2 vaccine: prolonged protection from SARS-CoV-2 in newborns and older children

Int J Infect Dis. 2022 Sep:122:905-909. doi: 10.1016/j.ijid.2022.06.055. Epub 2022 Jul 5.

Abstract

Objectives: Vaccination is the best strategy against COVID-19. We aimed to determine antibodies against SARS-CoV-2 in breastmilk and serum of mothers vaccinated with the mRNA vaccine.

Methods: This prospective study included 18 lactating women vaccinated with the BNT162b2 vaccine. Serum and breastmilk were collected before the first dose (T0), at the second dose (T1), 3 weeks after the second dose (T2), and 6 months after the first dose (T3). Serum anti-SARS-CoV-2 Spike (S) Immunoglobulin G (IgG) and Immunoglobulin A (IgA) were measured using a semi-quantitative enzyme-linked immunosorbent assay (ELISA) and secretory antibody (s) IgG and IgA in breastmilk using quantitative analysis.

Results: We detected serum anti-S IgG and IgA in all women after vaccination. Specific IgG and IgA were higher at T1, T2, and T3 compared with T0 (P <0.0001). Higher antibody levels were observed at T2 and lower values at T3 versus T2 (P = 0.007). After 6 months, all patients had serum IgG, but three of 18 (16%) had serum IgA. In breastmilk, sIgA was present at T1 and T2 and decreased after 6 months at T3 (P = 0.002). Breastmilk sIgG levels increased at T1 and T2 and peaked at T3 (P = 0.008).

Conclusion: Secretory antibodies were transmitted through breastmilk until 6 months after anti-COVID-19 mRNA vaccination. Protection of the newborn through breastfeeding needs to be addressed.

Keywords: Breastmilk; Immune response; Vaccine.

MeSH terms

  • Adolescent
  • Antibodies, Viral
  • BNT162 Vaccine
  • COVID-19* / prevention & control
  • Child
  • Female
  • Humans
  • Immunoglobulin A
  • Immunoglobulin G
  • Infant, Newborn
  • Lactation
  • Milk, Human
  • Prospective Studies
  • SARS-CoV-2
  • Vaccines*
  • Vaccines, Synthetic
  • mRNA Vaccines

Substances

  • Antibodies, Viral
  • Immunoglobulin A
  • Immunoglobulin G
  • Vaccines
  • Vaccines, Synthetic
  • mRNA Vaccines
  • BNT162 Vaccine