Characterizing collaborative transcription regulation with a graph-based deep learning approach

PLoS Comput Biol. 2022 Jun 6;18(6):e1010162. doi: 10.1371/journal.pcbi.1010162. eCollection 2022 Jun.

Abstract

Human epigenome and transcription activities have been characterized by a number of sequence-based deep learning approaches which only utilize the DNA sequences. However, transcription factors interact with each other, and their collaborative regulatory activities go beyond the linear DNA sequence. Therefore leveraging the informative 3D chromatin organization to investigate the collaborations among transcription factors is critical. We developed ECHO, a graph-based neural network, to predict chromatin features and characterize the collaboration among them by incorporating 3D chromatin organization from 200-bp high-resolution Micro-C contact maps. ECHO predicted 2,583 chromatin features with significantly higher average AUROC and AUPR than the best sequence-based model. We observed that chromatin contacts of different distances affected different types of chromatin features' prediction in diverse ways, suggesting complex and divergent collaborative regulatory mechanisms. Moreover, ECHO was interpretable via gradient-based attribution methods. The attributions on chromatin contacts identify important contacts relevant to chromatin features. The attributions on DNA sequences identify TF binding motifs and TF collaborative binding. Furthermore, combining the attributions on contacts and sequences reveals important sequence patterns in the neighborhood which are relevant to a target sequence's chromatin feature prediction.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Binding Sites / genetics
  • Chromatin / genetics
  • DNA* / chemistry
  • Deep Learning*
  • Gene Expression Regulation
  • Humans
  • Protein Binding
  • Transcription Factors / metabolism

Substances

  • Chromatin
  • Transcription Factors
  • DNA