Four species of grouper (family Epinephlidae), Red Hind (Epinephelus guttatus), Nassau (Epinephelus striatus), Black (Mycteroperca bonaci), and Yellowfin Grouper (Mycteroperca venenosa) share an aggregation site in Little Cayman, Cayman Islands and produce sounds while aggregating. Continuous observation of these aggregations is challenging because traditional diver or ship-based methods are limited in time and space. Passive acoustic localization can overcome this challenge for sound-producing species, allowing observations over long durations and at fine spatial scales. A hydrophone array was deployed in February 2017 over a 9-day period that included Nassau Grouper spawning. Passive acoustic localization was used to find positions of the grouper-produced calls recorded during this time, which enabled the measurement of call source levels and evaluation of spatiotemporal aspects of calling. Yellowfin Grouper had the lowest mean peak-to-peak (PP) call source level, and Nassau Grouper had the highest mean PP call source level (143.7 and 155.2 dB re: 1 μPa at 1 m for 70-170 Hz, respectively). During the days that Nassau Grouper spawned, calling peaked after sunset. Similarly, when Red Hind calls were abundant, calls were highest in the afternoon and evening. The measured source levels can be used to estimate communication and detection ranges and implement passive acoustic density estimation for these fishes.