A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

Haibo Xing; Wenchao Gu; Dang Xu; Fuxiang Tian; Linyun Yao; Zhenwei Wang; Xiaojun Hu

doi:10.1039/c7ra12970j

A simple fluorescent assay for cyromazine detection in raw milk by using CYR-stabilized G-quadruplex formation

RSC Adv. 2018 Jan 10;8(5):2418-2425. doi: 10.1039/c7ra12970j. eCollection 2018 Jan 9.

Authors

Haibo Xing¹, Wenchao Gu², Dang Xu¹, Fuxiang Tian¹, Linyun Yao³, Zhenwei Wang¹, Xiaojun Hu¹

Affiliations

¹ School of Chemical and Environmental Engineering, Shanghai Institute of Technology Shanghai 201418 China 267358918@qq.com.
² Putuo District Center for Disease Control and Prevention Shanghai China.
³ School of Perfume and Aroma Technology, Shanghai Institute of Technology Shanghai 201418 China.

Abstract

A rapid biosensor for the detection of cyromazine in milk is reported based on a fluorescence quenching result. When an FAM labelled G-rich ssDNA Tcy2 is treated with cyromazine, it can form a G-quadruplex-CYR complex and cause a change in fluorescence. As a result, the presence of cyromazine can be determined by fluorescence quenching. This sensor is selective for the detection of cyromazine in raw milk and has a limit of detection of 0.68 ppb and a detection range from 0 to 200 ppb.