Nonlesional lupus skin contributes to inflammatory education of myeloid cells and primes for cutaneous inflammation

Sci Transl Med. 2022 Apr 27;14(642):eabn2263. doi: 10.1126/scitranslmed.abn2263. Epub 2022 Apr 27.

Abstract

Cutaneous lupus erythematosus (CLE) is a disfiguring and poorly understood condition frequently associated with systemic lupus. Previous studies suggest that nonlesional keratinocytes play a role in disease predisposition, but this has not been investigated in a comprehensive manner or in the context of other cell populations. To investigate CLE immunopathogenesis, normal-appearing skin, lesional skin, and circulating immune cells from lupus patients were analyzed via integrated single-cell RNA sequencing and spatial RNA sequencing. We demonstrate that normal-appearing skin of patients with lupus represents a type I interferon-rich, prelesional environment that skews gene transcription in all major skin cell types and markedly distorts predicted cell-cell communication networks. We also show that lupus-enriched CD16+ dendritic cells undergo robust interferon education in the skin, thereby gaining proinflammatory phenotypes. Together, our data provide a comprehensive characterization of lesional and nonlesional skin in lupus and suggest a role for skin education of CD16+ dendritic cells in CLE pathogenesis.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Humans
  • Inflammation / pathology
  • Interferon Type I* / metabolism
  • Keratinocytes / pathology
  • Lupus Erythematosus, Cutaneous*
  • Myeloid Cells / metabolism

Substances

  • Interferon Type I