Malaria diagnostic methods with the elimination goal in view

Parasitol Res. 2022 Jul;121(7):1867-1885. doi: 10.1007/s00436-022-07512-9. Epub 2022 Apr 23.

Abstract

Malaria control measures have been in use for years but have not completely curbed the spread of infection. Ultimately, global elimination is the goal. A major playmaker in the various approaches to reaching the goal is the issue of proper diagnosis. Various diagnostic techniques were adopted in different regions and geographical locations over the decades, and these have invariably produced diverse outcomes. In this review, we looked at the various approaches used in malaria diagnostics with a focus on methods favorably used during pre-elimination and elimination phases as well as in endemic regions. Microscopy, rapid diagnostic testing (RDT), loop-mediated isothermal amplification (LAMP), and polymerase chain reaction (PCR) are common methods applied depending on prevailing factors, each with its strengths and limitations. As the drive toward the elimination goal intensifies, the search for ideal, simple, fast, and reliable point-of-care diagnostic tools is needed more than ever before to be used in conjunction with a functional surveillance system supported by the ideal vaccine.

Keywords: Diagnostics; Elimination; Malaria; Microscopy; RDT; Surveillance; Vaccine.

Publication types

  • Review

MeSH terms

  • Diagnostic Tests, Routine / methods
  • Goals
  • Humans
  • Malaria* / diagnosis
  • Malaria* / prevention & control
  • Malaria, Falciparum* / epidemiology
  • Microscopy / methods
  • Molecular Diagnostic Techniques / methods
  • Nucleic Acid Amplification Techniques / methods
  • Polymerase Chain Reaction / methods
  • Sensitivity and Specificity